Detailed information of TA system TA206442

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 20604..21023
• Replicon: plasmid unnamed3
• Accession: NZ_CP076712
• Organism: Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: KQ220_RS24935
• Protein ID: WP_223200913.1
• Coordinates: 20901..21023 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	20604..20816 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ220_RS24900 (16596)	16596..17159	+	564	WP_059320955.1	class I SAM-dependent methyltransferase	-
KQ220_RS25510 (17140)	17140..17689	-	550	Protein_27	hypothetical protein	-
KQ220_RS24905 (17759)	17759..17965	+	207	WP_000275856.1	hypothetical protein	-
KQ220_RS24910 (17991)	17991..18518	+	528	WP_000290820.1	single-stranded DNA-binding protein	-
KQ220_RS24915 (18576)	18576..18809	+	234	WP_000006001.1	DUF905 family protein	-
KQ220_RS24920 (18950)	18950..19387	+	438	Protein_31	hypothetical protein	-
KQ220_RS24925 (19442)	19442..19876	+	435	WP_059320953.1	conjugation system SOS inhibitor PsiB	-
KQ220_RS24930 (19873)	19873..20614	+	742	Protein_33	plasmid SOS inhibition protein A	-
- (20604)	20604..20816	+	213	NuclAT_0	-	Antitoxin
- (20604)	20604..20816	+	213	NuclAT_0	-	Antitoxin
- (20604)	20604..20816	+	213	NuclAT_0	-	Antitoxin
- (20604)	20604..20816	+	213	NuclAT_0	-	Antitoxin
KQ220_RS25515 (20804)	20804..20956	+	153	Protein_34	DUF5431 family protein	-
KQ220_RS24935 (20901)	20901..21023	+	123	WP_223200913.1	Hok/Gef family protein	Toxin
KQ220_RS24940 (21262)	21262..21622	-	361	Protein_36	hypothetical protein	-
KQ220_RS24945 (21692)	21692..21898	+	207	WP_000275856.1	hypothetical protein	-
KQ220_RS24950 (21922)	21922..22224	+	303	WP_001272235.1	hypothetical protein	-
KQ220_RS24955 (22380)	22380..22667	+	288	WP_000107538.1	hypothetical protein	-
KQ220_RS24960 (22785)	22785..23606	+	822	Protein_40	DUF932 domain-containing protein	-
KQ220_RS24965 (23903)	23903..24505	-	603	WP_225932714.1	transglycosylase SLT domain-containing protein	-
KQ220_RS24970 (24826)	24826..25209	+	384	WP_001151537.1	conjugal transfer relaxosome DNA-binding protein TraM	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..66958	66958

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T206442 WP_223200913.1 NZ_CP076712:20901-21023 [Escherichia coli O158:H23]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T206442 NZ_CP102391:c119107-118664 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
TGGGCACGCTATCGCGGCCGGCGCCGTCCTAGTGACGAACAATGTGAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGAAAGCCGCTCTCTGCAGGGTAATTTCTTGA

Antitoxin

Nucleotide

• Length: 213 bp

>AT206442 NZ_CP076712:20604-20816 [Escherichia coli O158:H23]
TCACACGGATTACCCGTAGCCTGAATGAGCTGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGCGCAGGGATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin