Detailed information of TA system TA206438

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 56682..56935
• Replicon: plasmid unnamed2
• Accession: NZ_CP076711
• Organism: Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: KQ220_RS24685
• Protein ID: WP_106906997.1
• Coordinates: 56682..56831 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	56876..56935 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ220_RS24655 (52907)	52907..53311	-	405	WP_000154256.1	DUF2251 domain-containing protein	-
KQ220_RS24660 (53481)	53481..53768	-	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KQ220_RS24665 (53765)	53765..54016	-	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
KQ220_RS24670 (54986)	54986..55843	-	858	WP_158709091.1	incFII family plasmid replication initiator RepA	-
KQ220_RS25490 (55862)	55862..56038	-	177	Protein_67	protein CopA/IncA	-
KQ220_RS24680 (56141)	56141..56398	-	258	WP_106906995.1	replication regulatory protein RepA	-
KQ220_RS24685 (56682)	56682..56831	-	150	WP_106906997.1	Hok/Gef family protein	Toxin
- (56876)	56876..56935	+	60	NuclAT_0	-	Antitoxin
- (56876)	56876..56935	+	60	NuclAT_0	-	Antitoxin
- (56876)	56876..56935	+	60	NuclAT_0	-	Antitoxin
- (56876)	56876..56935	+	60	NuclAT_0	-	Antitoxin
KQ220_RS24690 (57101)	57101..59245	-	2145	WP_106907000.1	peptidase domain-containing ABC transporter	-
KQ220_RS24695 (59242)	59242..59619	-	378	WP_216781565.1	HlyD family efflux transporter periplasmic adaptor subunit	-
KQ220_RS24700 (59619)	59619..60521	-	903	WP_216781566.1	biotin/lipoyl-binding protein	-
KQ220_RS24705 (60900)	60900..61475	-	576	WP_106907004.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..69043	69043

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5515.64 Da
• Isoelectric Point: 8.7678

>T206438 WP_106906997.1 NZ_CP076711:c56831-56682 [Escherichia coli O158:H23]
MTKYALIGLLAVCATVLCFSLIFRERLCELSIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T206438 NZ_CP102390:c4949464-4948547 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGTGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 60 bp

>AT206438 NZ_CP076711:56876-56935 [Escherichia coli O158:H23]
AACGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin