Detailed information of TA system TA206432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3580171..3580970
• Replicon: chromosome
• Accession: NZ_CP076709
• Organism: Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: A0A1M2ER85
• Locus tag: KQ220_RS17265
• Protein ID: WP_032352641.1
• Coordinates: 3580506..3580970 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A271UK16
• Locus tag: KQ220_RS17260
• Protein ID: WP_033869832.1
• Coordinates: 3580171..3580506 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ220_RS17245 (3575962)	3575962..3576726	-	765	WP_112005242.1	2-dehydro-3-deoxyglucarate aldolase	-
KQ220_RS17250 (3576742)	3576742..3578076	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
KQ220_RS17255 (3578451)	3578451..3580022	+	1572	WP_001273738.1	galactarate dehydratase	-
KQ220_RS17260 (3580171)	3580171..3580506	+	336	WP_033869832.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
KQ220_RS17265 (3580506)	3580506..3580970	+	465	WP_032352641.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
KQ220_RS17270 (3581025)	3581025..3581834	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
KQ220_RS17275 (3582083)	3582083..3583363	+	1281	WP_000681955.1	tagatose-bisphosphate aldolase subunit KbaZ	-
KQ220_RS17280 (3583386)	3583386..3583859	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
KQ220_RS17285 (3583870)	3583870..3584649	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
KQ220_RS17290 (3584639)	3584639..3585517	+	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
KQ220_RS17295 (3585535)	3585535..3585969	+	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3569802..3580970	11168

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17814.20 Da
• Isoelectric Point: 9.4942

>T206432 WP_032352641.1 NZ_CP076709:3580506-3580970 [Escherichia coli O158:H23]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETD

Nucleotide

• Length: 465 bp

>T206432 NZ_CP102390:884829-885236 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCGTGC
GCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCCGAA
TGGGAAATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGGACA
GCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGGCGC
AGGAGTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12330.97 Da
• Isoelectric Point: 4.8616

>AT206432 WP_033869832.1 NZ_CP076709:3580171-3580506 [Escherichia coli O158:H23]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNT
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT206432 NZ_CP102390:884582-884848 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A1M2ER85

Antitoxin

• AlphaFold DB: A0A271UK16