Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3094113..3094840 Replicon chromosome
Accession NZ_CP076709
Organism Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)


Gene name higB Uniprot ID D6JE58
Locus tag KQ220_RS14955 Protein ID WP_000547557.1
Coordinates 3094529..3094840 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1M2G9D6
Locus tag KQ220_RS14950 Protein ID WP_001600394.1
Coordinates 3094113..3094532 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ220_RS14930 (3089198) 3089198..3089725 - 528 WP_001078777.1 electron transport protein HydN -
KQ220_RS14935 (3089874) 3089874..3090884 - 1011 WP_024211331.1 DNA-binding transcriptional regulator AscG -
KQ220_RS14940 (3091144) 3091144..3092601 + 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
KQ220_RS14945 (3092610) 3092610..3094034 + 1425 WP_216781204.1 6-phospho-beta-glucosidase AscB -
KQ220_RS14950 (3094113) 3094113..3094532 - 420 WP_001600394.1 helix-turn-helix domain-containing protein Antitoxin
KQ220_RS14955 (3094529) 3094529..3094840 - 312 WP_000547557.1 type II toxin-antitoxin system HigB family toxin Toxin
KQ220_RS14960 (3095007) 3095007..3095477 - 471 WP_000132947.1 hydrogenase maturation peptidase HycI -
KQ220_RS14965 (3095470) 3095470..3095880 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
KQ220_RS14970 (3095877) 3095877..3096644 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
KQ220_RS14975 (3096644) 3096644..3097186 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
KQ220_RS14980 (3097196) 3097196..3098905 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12467.20 Da        Isoelectric Point: 9.5262

>T206430 WP_000547557.1 NZ_CP076709:c3094840-3094529 [Escherichia coli O158:H23]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T206430 NZ_CP102390:356488-356835 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15389.40 Da        Isoelectric Point: 4.4596

>AT206430 WP_001600394.1 NZ_CP076709:c3094532-3094113 [Escherichia coli O158:H23]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYESNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT206430 NZ_CP102390:356835-357134 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB D6JE58

Antitoxin

Source ID Structure
AlphaFold DB A0A1M2G9D6

References