20643

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) BrnTA/BrnT_toxin-BrnA
Location 684058..684635 Replicon chromosome
Accession NC_003047
Organism Sinorhizobium meliloti 1021

Toxin (Protein)


Gene name brnT Uniprot ID Q92S20
Locus tag SM_RS03140 Protein ID WP_010968707.1
Coordinates 684058..684321 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name brnA Uniprot ID Q92KN5
Locus tag SM_RS03145 Protein ID WP_010968708.1
Coordinates 684318..684635 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SM_RS03100 (SMc02306) 679388..679828 + 441 WP_010968702.1 DUF2948 family protein -
SM_RS03105 (SMc02307) 679893..681197 + 1305 WP_010968703.1 histidinol dehydrogenase -
SM_RS03110 (SMc02308) 681194..681670 + 477 WP_010968704.1 UPF0262 family protein -
SM_RS03115 (SMc02309) 681684..682163 + 480 WP_010968705.1 low molecular weight phosphatase family protein -
SM_RS03120 (SMc02310) 682321..682539 + 219 WP_004435948.1 translation initiation factor IF-1 -
SM_RS03125 (SMc02311) 682637..683257 + 621 WP_004435951.1 Maf-like protein -
SM_RS03130 (SMc02312) 683262..683474 + 213 WP_010968706.1 DNA gyrase inhibitor YacG -
SM_RS03140 (SMc04431) 684058..684321 + 264 WP_010968707.1 BrnT family toxin Toxin
SM_RS03145 (SMc04432) 684318..684635 + 318 WP_010968708.1 BrnA antitoxin family protein Antitoxin
SM_RS03150 (SMc02313) 684838..685302 - 465 WP_013844019.1 type II toxin-antitoxin system HicB family antitoxin -
SM_RS03155 685299..685487 - 189 WP_013844020.1 hypothetical protein -
SM_RS03160 (SMc02314) 685807..686907 + 1101 WP_010968710.1 hypothetical protein -
SM_RS03165 (SMc02315) 686894..687976 + 1083 WP_010968711.1 nucleotidyltransferase family protein -
SM_RS03170 (SMc02316) 688179..688490 + 312 WP_004435964.1 hypothetical protein -
SM_RS03175 (SMc02317) 688770..689159 - 390 WP_010968712.1 DUF930 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 652921..685487 32566


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(5-82)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(26-103)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 9821.16 Da        Isoelectric Point: 10.3413

>T20643 WP_010968707.1 NC_003047:684058-684321 [Sinorhizobium meliloti 1021]
MRIVWGEPKRRANLEKHGFDFADVSDLDWASALIEDSKPDTSGRKRLKAIGYFRDGTAAVIFATLGTEAISIISFRPASD
RERRRLP

Download         Length: 264 bp

>T20643 NC_003047:684058-684321 [Sinorhizobium meliloti 1021]
ATGCGAATTGTGTGGGGCGAACCCAAACGGCGAGCCAACCTTGAGAAGCATGGTTTCGATTTTGCCGATGTAAGCGACCT
CGATTGGGCGAGTGCCCTCATAGAGGACAGCAAGCCAGATACTTCTGGCAGAAAGCGCCTGAAGGCTATCGGTTATTTTC
GTGACGGGACGGCAGCGGTCATTTTCGCGACACTCGGAACGGAAGCTATATCGATCATCAGCTTTCGTCCTGCAAGCGAC
AGAGAAAGAAGGAGGCTTCCATGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12197.83 Da        Isoelectric Point: 10.0883

>AT20643 WP_010968708.1 NC_003047:684318-684635 [Sinorhizobium meliloti 1021]
MTTRTRKSKTFEPGHGYTKEEWDAVDFPELTDEELTNMRPARDVLPPEFFKAMEEHRRSRGRPSLEHPKKQVTLRLDEDV
IAKFRAGGKGWQSRMNEALRKAAGI

Download         Length: 318 bp

>AT20643 NC_003047:684318-684635 [Sinorhizobium meliloti 1021]
ATGACTACCAGGACCCGAAAATCGAAAACGTTCGAGCCAGGTCACGGCTATACAAAAGAGGAATGGGATGCAGTCGATTT
TCCCGAGTTGACCGATGAAGAGCTTACCAACATGCGTCCCGCGAGAGATGTGCTGCCGCCTGAGTTTTTCAAAGCTATGG
AGGAGCATCGCAGATCGCGTGGGCGCCCCTCGCTGGAGCATCCGAAAAAGCAAGTCACCCTCCGGTTGGACGAAGACGTG
ATCGCTAAGTTTCGAGCTGGTGGCAAAGGCTGGCAGAGCCGGATGAATGAGGCGCTGCGAAAAGCTGCAGGTATATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q92S20

Antitoxin

Source ID Structure
AlphaFold DB Q92KN5

References