Detailed information of TA system TA206429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2306254..2306756
• Replicon: chromosome
• Accession: NZ_CP076709
• Organism: Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: -
• Locus tag: KQ220_RS11235
• Protein ID: WP_112005156.1
• Coordinates: 2306254..2306508 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: KQ220_RS11240
• Protein ID: WP_001259255.1
• Coordinates: 2306505..2306756 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ220_RS11205 (2301269)	2301269..2301496	-	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-
KQ220_RS11210 (2301510)	2301510..2302568	-	1059	WP_000492310.1	thiosulfate utilization transporter TsuA/YeeE	-
KQ220_RS11215 (2302747)	2302747..2304105	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
KQ220_RS11220 (2304095)	2304095..2304157	-	63	WP_010723108.1	membrane protein YoeI	-
KQ220_RS11225 (2304372)	2304372..2305301	-	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
KQ220_RS11230 (2305347)	2305347..2306171	-	825	WP_000754737.1	SDR family oxidoreductase	-
KQ220_RS11235 (2306254)	2306254..2306508	-	255	WP_112005156.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KQ220_RS11240 (2306505)	2306505..2306756	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KQ220_RS11245 (2307039)	2307039..2307089	+	51	WP_001364200.1	his operon leader peptide	-
KQ220_RS11250 (2307235)	2307235..2308134	+	900	WP_000131789.1	ATP phosphoribosyltransferase	-
KQ220_RS11255 (2308140)	2308140..2309444	+	1305	WP_001376955.1	histidinol dehydrogenase	-
KQ220_RS11260 (2309441)	2309441..2310511	+	1071	WP_000108943.1	histidinol-phosphate transaminase	-
KQ220_RS11265 (2310511)	2310511..2311578	+	1068	WP_001544685.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10171.53 Da
• Isoelectric Point: 8.0353

>T206429 WP_112005156.1 NZ_CP076709:c2306508-2306254 [Escherichia coli O158:H23]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRTTEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T206429 NZ_CP102390:330517-330885 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT206429 WP_001259255.1 NZ_CP076709:c2306756-2306505 [Escherichia coli O158:H23]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT206429 NZ_CP102390:330299-330520 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0