Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1676095..1676657 Replicon chromosome
Accession NZ_CP076709
Organism Escherichia coli O158:H23 strain CFIAFB20140388

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag KQ220_RS07940 Protein ID WP_000605676.1
Coordinates 1676379..1676657 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag KQ220_RS07935 Protein ID WP_000781370.1
Coordinates 1676095..1676379 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ220_RS07920 (1671453) 1671453..1674500 + 3048 WP_012767733.1 formate dehydrogenase-N subunit alpha -
KQ220_RS07925 (1674513) 1674513..1675397 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
KQ220_RS07930 (1675390) 1675390..1676043 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
KQ220_RS07935 (1676095) 1676095..1676379 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
KQ220_RS07940 (1676379) 1676379..1676657 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KQ220_RS07945 (1676842) 1676842..1677852 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
KQ220_RS07950 (1677986) 1677986..1679683 - 1698 WP_000433464.1 malate dehydrogenase -
KQ220_RS07955 (1679840) 1679840..1679977 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
KQ220_RS07960 (1680079) 1680079..1680294 - 216 WP_000495766.1 biofilm-dependent modulation protein -
KQ220_RS07965 (1680639) 1680639..1681070 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T206419 WP_000605676.1 NZ_CP076709:c1676657-1676379 [Escherichia coli O158:H23]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T206419 NZ_CP102380:3148441-3148905 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT206419 WP_000781370.1 NZ_CP076709:c1676379-1676095 [Escherichia coli O158:H23]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT206419 NZ_CP102380:3148106-3148441 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References