Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3840652..3841346 Replicon chromosome
Accession NZ_CP076706
Organism Escherichia coli O170:H18 strain CFIAFB20120266

Toxin (Protein)


Gene name yafO Uniprot ID B7MQ69
Locus tag KQ217_RS18605 Protein ID WP_001263501.1
Coordinates 3840948..3841346 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag KQ217_RS18600 Protein ID WP_000554755.1
Coordinates 3840652..3840945 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ217_RS18575 (3836466) 3836466..3836933 + 468 WP_000725264.1 flagellar basal body-associated FliL family protein -
KQ217_RS18580 (3836953) 3836953..3837672 + 720 WP_000938737.1 FliA/WhiG family RNA polymerase sigma factor -
KQ217_RS18585 (3837685) 3837685..3838548 + 864 WP_001169519.1 flagellar motor stator protein MotA -
KQ217_RS18590 (3838551) 3838551..3839474 + 924 WP_001232551.1 putative lateral flagellar export/assembly protein LafU -
KQ217_RS18595 (3839545) 3839545..3840600 + 1056 WP_001226167.1 DNA polymerase IV -
KQ217_RS18600 (3840652) 3840652..3840945 + 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KQ217_RS18605 (3840948) 3840948..3841346 + 399 WP_001263501.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KQ217_RS18610 (3841356) 3841356..3841808 + 453 WP_001059895.1 GNAT family N-acetyltransferase -
KQ217_RS18615 (3841998) 3841998..3843137 + 1140 WP_000521577.1 RNA ligase RtcB family protein -
KQ217_RS18620 (3843134) 3843134..3843748 + 615 WP_000602123.1 peptide chain release factor H -
KQ217_RS18625 (3843805) 3843805..3845262 - 1458 WP_001292999.1 cytosol nonspecific dipeptidase -
KQ217_RS18630 (3845523) 3845523..3845981 + 459 WP_001291991.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0950

>T206405 WP_001263501.1 NZ_CP076706:3840948-3841346 [Escherichia coli O170:H18]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T206405 NZ_CP102380:c1286367-1286173 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT206405 WP_000554755.1 NZ_CP076706:3840652-3840945 [Escherichia coli O170:H18]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT206405 NZ_CP102380:1285996-1286174 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376DBU4

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References