Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3429746..3430274 Replicon chromosome
Accession NZ_CP076706
Organism Escherichia coli O170:H18 strain CFIAFB20120266

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag KQ217_RS24720 Protein ID WP_229377364.1
Coordinates 3430044..3430274 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KQ217_RS24715 Protein ID WP_101973818.1
Coordinates 3429746..3430054 (-) Length 103 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ217_RS16530 (3424839) 3424839..3425201 - 363 WP_001242716.1 hypothetical protein -
KQ217_RS16535 (3425192) 3425192..3425728 - 537 WP_000008178.1 5'-deoxynucleotidase -
KQ217_RS16540 (3425856) 3425856..3426680 - 825 WP_000081287.1 DUF2303 family protein -
KQ217_RS16545 (3426746) 3426746..3427108 - 363 WP_216803487.1 hypothetical protein -
KQ217_RS16550 (3427811) 3427811..3428503 - 693 WP_001311077.1 XRE family transcriptional regulator -
KQ217_RS16555 (3428601) 3428601..3428861 + 261 WP_001191669.1 helix-turn-helix transcriptional regulator -
KQ217_RS16560 (3428854) 3428854..3429405 + 552 WP_101973819.1 protein YmfL -
KQ217_RS24710 (3429551) 3429551..3429724 + 174 WP_071829521.1 DUF4222 domain-containing protein -
KQ217_RS24715 (3429746) 3429746..3430054 - 309 WP_101973818.1 helix-turn-helix transcriptional regulator Antitoxin
KQ217_RS24720 (3430044) 3430044..3430274 - 231 WP_229377364.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KQ217_RS24725 (3430239) 3430239..3431261 + 1023 WP_229377367.1 Rha family phage regulatory protein -
KQ217_RS16570 (3431258) 3431258..3431452 + 195 WP_216803488.1 hypothetical protein -
KQ217_RS16575 (3431449) 3431449..3431673 + 225 WP_101973816.1 hypothetical protein -
KQ217_RS16580 (3431670) 3431670..3432488 + 819 WP_101973815.1 helix-turn-helix domain-containing protein -
KQ217_RS16585 (3432485) 3432485..3432979 + 495 WP_021576994.1 PerC family transcriptional regulator -
KQ217_RS16590 (3432979) 3432979..3433305 + 327 WP_000210178.1 LexA repressor -
KQ217_RS16595 (3433302) 3433302..3433667 + 366 WP_044717961.1 RusA family crossover junction endodeoxyribonuclease -
KQ217_RS16600 (3433849) 3433849..3434664 + 816 WP_101973814.1 KilA-N domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - stx2A / stx2B 3419889..3471306 51417


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(5-70)

Antitoxin

Cro/C1-type helix-turn-helix domain

(44-94)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8680.17 Da        Isoelectric Point: 10.5308

>T206401 WP_229377364.1 NZ_CP076706:c3430274-3430044 [Escherichia coli O170:H18]
MKVYGSNLGEPHTSAFGDGLFELRIKGSDGIARVFYCTLAGKRIIMLHSFIKKTQKTPPVERKKAETRMKEVKHDW

Download         Length: 231 bp

>T206401 NZ_CP102380:c366492-366274 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 103 a.a.        Molecular weight: 11154.84 Da        Isoelectric Point: 8.5840

>AT206401 WP_101973818.1 NZ_CP076706:c3430054-3429746 [Escherichia coli O170:H18]
MTGKRNPPTMTHDEMAAKWMDDPAFKAEYDAIADEFALLDEMLAARKEAGLTQAEVAERMGTKATAITRMESNLASGVSG
PSFATLKKFARATGKKLQIRFV

Download         Length: 309 bp

>AT206401 NZ_CP102380:c366892-366518 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References