Detailed information of TA system TA206397

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-orzP/SymE(toxin)
• Location: 3387160..3387572
• Replicon: chromosome
• Accession: NZ_CP076706
• Organism: Escherichia coli O170:H18 strain CFIAFB20120266

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A5E9Z711
• Locus tag: KQ217_RS16350
• Protein ID: WP_000132600.1
• Coordinates: 3387160..3387501 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	orzP
Locus tag	-
Coordinates	3387496..3387572 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ217_RS16325 (3382229)	3382229..3383509	-	1281	WP_033806827.1	DUF445 domain-containing protein	-
KQ217_RS16330 (3383694)	3383694..3384614	+	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
KQ217_RS16335 (3384842)	3384842..3384922	+	81	WP_020233658.1	hypothetical protein	-
KQ217_RS16340 (3385020)	3385020..3385902	+	883	Protein_3193	DUF262 domain-containing protein	-
KQ217_RS16345 (3385950)	3385950..3387113	+	1164	WP_216803883.1	DUF1524 domain-containing protein	-
KQ217_RS16350 (3387160)	3387160..3387501	-	342	WP_000132600.1	endoribonuclease SymE	Toxin
- (3387496)	3387496..3387572	+	77	NuclAT_5	-	Antitoxin
- (3387496)	3387496..3387572	+	77	NuclAT_5	-	Antitoxin
- (3387496)	3387496..3387572	+	77	NuclAT_5	-	Antitoxin
- (3387496)	3387496..3387572	+	77	NuclAT_5	-	Antitoxin
- (3387496)	3387496..3387572	+	77	NuclAT_6	-	Antitoxin
- (3387496)	3387496..3387572	+	77	NuclAT_6	-	Antitoxin
- (3387496)	3387496..3387572	+	77	NuclAT_6	-	Antitoxin
- (3387496)	3387496..3387572	+	77	NuclAT_6	-	Antitoxin
KQ217_RS16355 (3387722)	3387722..3389476	-	1755	WP_021536045.1	restriction endonuclease subunit S	-
KQ217_RS16360 (3389476)	3389476..3390945	-	1470	WP_216803481.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	3385671..3404085	18414

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12251.10 Da
• Isoelectric Point: 9.0999

>T206397 WP_000132600.1 NZ_CP076706:c3387501-3387160 [Escherichia coli O170:H18]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLAAAGFATGTAIDVKVMEGCIVLTAQPTAAEESE
LMQSLRQVCKLSARKQKQVQEFIGVITGKKKVA

Nucleotide

• Length: 342 bp

>T206397 NZ_CP102379:4324477-4324827 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT206397 NZ_CP076706:3387496-3387572 [Escherichia coli O170:H18]
AGTCATAACTGCTATTCTCCTGGAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A5E9Z711

Antitoxin