Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/HTH(antitoxin) |
| Location | 2878415..2879017 | Replicon | chromosome |
| Accession | NZ_CP076706 | ||
| Organism | Escherichia coli O170:H18 strain CFIAFB20120266 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | S1P416 |
| Locus tag | KQ217_RS13995 | Protein ID | WP_000897302.1 |
| Coordinates | 2878415..2878726 (+) | Length | 104 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | KQ217_RS14000 | Protein ID | WP_000356397.1 |
| Coordinates | 2878727..2879017 (+) | Length | 97 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KQ217_RS13965 (2873445) | 2873445..2874230 | + | 786 | WP_000059678.1 | DeoR/GlpR family DNA-binding transcription regulator | - |
| KQ217_RS13970 (2874329) | 2874329..2874928 | + | 600 | WP_001314324.1 | glucose-1-phosphatase | - |
| KQ217_RS13975 (2874922) | 2874922..2875794 | + | 873 | WP_000920762.1 | virulence factor BrkB family protein | - |
| KQ217_RS13980 (2875791) | 2875791..2876228 | + | 438 | WP_000560978.1 | D-aminoacyl-tRNA deacylase | - |
| KQ217_RS13985 (2876273) | 2876273..2877214 | + | 942 | WP_001297068.1 | fatty acid biosynthesis protein FabY | - |
| KQ217_RS13990 (2877278) | 2877278..2878186 | - | 909 | WP_001331553.1 | alpha/beta hydrolase | - |
| KQ217_RS13995 (2878415) | 2878415..2878726 | + | 312 | WP_000897302.1 | hypothetical protein | Toxin |
| KQ217_RS14000 (2878727) | 2878727..2879017 | + | 291 | WP_000356397.1 | NadS family protein | Antitoxin |
| KQ217_RS14005 (2879352) | 2879352..2880374 | + | 1023 | WP_001455283.1 | IS21-like element IS100 family transposase | - |
| KQ217_RS14010 (2880371) | 2880371..2881153 | + | 783 | WP_001317493.1 | IS21-like element IS100kyp family helper ATPase IstB | - |
| KQ217_RS14015 (2881335) | 2881335..2881613 | + | 279 | WP_001296612.1 | hypothetical protein | - |
| KQ217_RS14020 (2882009) | 2882009..2882227 | + | 219 | WP_001314326.1 | CopG family transcriptional regulator | - |
| KQ217_RS14025 (2882443) | 2882443..2883372 | - | 930 | WP_000027720.1 | formate dehydrogenase accessory protein FdhE | - |
| KQ217_RS14030 (2883369) | 2883369..2884004 | - | 636 | WP_000829013.1 | formate dehydrogenase cytochrome b556 subunit | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 104 a.a. Molecular weight: 12203.19 Da Isoelectric Point: 9.7791
>T206395 WP_000897302.1 NZ_CP076706:2878415-2878726 [Escherichia coli O170:H18]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW
Download Length: 312 bp
>T206395 NZ_CP102379:3147105-3147569 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA
Antitoxin
Download Length: 97 a.a. Molecular weight: 10846.43 Da Isoelectric Point: 10.8907
>AT206395 WP_000356397.1 NZ_CP076706:2878727-2879017 [Escherichia coli O170:H18]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR
Download Length: 291 bp
>AT206395 NZ_CP102379:3146770-3147105 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|