Detailed information of TA system TA206394

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2063429..2064228
• Replicon: chromosome
• Accession: NZ_CP076706
• Organism: Escherichia coli O170:H18 strain CFIAFB20120266

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: KQ217_RS10040
• Protein ID: WP_000347251.1
• Coordinates: 2063764..2064228 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: KQ217_RS10035
• Protein ID: WP_001296435.1
• Coordinates: 2063429..2063764 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ217_RS10020 (2059214)	2059214..2059984	-	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-
KQ217_RS10025 (2060000)	2060000..2061334	-	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
KQ217_RS10030 (2061709)	2061709..2063280	+	1572	WP_001273939.1	galactarate dehydratase	-
KQ217_RS10035 (2063429)	2063429..2063764	+	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
KQ217_RS10040 (2063764)	2063764..2064228	+	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
KQ217_RS10045 (2064283)	2064283..2065092	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
KQ217_RS10050 (2065341)	2065341..2066621	+	1281	WP_000681941.1	tagatose-bisphosphate aldolase subunit KbaZ	-
KQ217_RS10055 (2066644)	2066644..2067117	+	474	WP_001298322.1	PTS N-acetylgalactosamine transporter subunit IIB	-
KQ217_RS10060 (2067128)	2067128..2067907	+	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
KQ217_RS10065 (2067897)	2067897..2068775	+	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
KQ217_RS10070 (2068793)	2068793..2069227	+	435	WP_000948840.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T206394 WP_000347251.1 NZ_CP076706:2063764-2064228 [Escherichia coli O170:H18]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T206394 NZ_CP102379:c3104222-3103908 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT206394 WP_001296435.1 NZ_CP076706:2063429-2063764 [Escherichia coli O170:H18]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT206394 NZ_CP102379:c3103911-3103495 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: S1PPV5