Detailed information of TA system TA206391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1591036..1591763
• Replicon: chromosome
• Accession: NZ_CP076706
• Organism: Escherichia coli O170:H18 strain CFIAFB20120266

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: KQ217_RS07850
• Protein ID: WP_000547563.1
• Coordinates: 1591452..1591763 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KQ217_RS07845
• Protein ID: WP_000126304.1
• Coordinates: 1591036..1591455 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ217_RS07830 (1587159)	1587159..1589408	-	2250	WP_216803811.1	carbamoyltransferase HypF	-
KQ217_RS07835 (1589561)	1589561..1590088	-	528	WP_001078777.1	electron transport protein HydN	-
KQ217_RS07840 (1590516)	1590516..1590944	+	429	WP_000536067.1	hypothetical protein	-
KQ217_RS07845 (1591036)	1591036..1591455	-	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
KQ217_RS07850 (1591452)	1591452..1591763	-	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
KQ217_RS07855 (1591926)	1591926..1592681	-	756	WP_001553332.1	hypothetical protein	-
KQ217_RS07860 (1592724)	1592724..1593194	-	471	WP_216803812.1	hydrogenase maturation peptidase HycI	-
KQ217_RS07865 (1593187)	1593187..1593597	-	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
KQ217_RS07870 (1593594)	1593594..1594361	-	768	WP_000067401.1	formate hydrogenlyase subunit HycG	-
KQ217_RS07875 (1594361)	1594361..1594903	-	543	WP_000493797.1	formate hydrogenlyase subunit HycF	-
KQ217_RS07880 (1594913)	1594913..1596622	-	1710	WP_001288136.1	formate hydrogenlyase subunit HycE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T206391 WP_000547563.1 NZ_CP076706:c1591763-1591452 [Escherichia coli O170:H18]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T206391 NZ_CP102379:c2780859-2780524 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT206391 WP_000126304.1 NZ_CP076706:c1591455-1591036 [Escherichia coli O170:H18]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT206391 NZ_CP102379:c2781107-2780859 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22