Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 206558..207120 Replicon chromosome
Accession NZ_CP076706
Organism Escherichia coli O170:H18 strain CFIAFB20120266

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag KQ217_RS00980 Protein ID WP_000605675.1
Coordinates 206842..207120 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag KQ217_RS00975 Protein ID WP_000781370.1
Coordinates 206558..206842 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ217_RS00960 (201739) 201739..204786 + 3048 WP_015912497.1 formate dehydrogenase-N subunit alpha -
KQ217_RS00965 (204799) 204799..205683 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
KQ217_RS00970 (205676) 205676..206329 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
KQ217_RS00975 (206558) 206558..206842 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
KQ217_RS00980 (206842) 206842..207120 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KQ217_RS00985 (207306) 207306..208316 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
KQ217_RS00990 (208450) 208450..210147 - 1698 WP_000433464.1 malate dehydrogenase -
KQ217_RS00995 (210304) 210304..210441 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
KQ217_RS01000 (210543) 210543..210758 - 216 WP_000495766.1 biofilm-dependent modulation protein -
KQ217_RS01005 (211103) 211103..211534 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T206379 WP_000605675.1 NZ_CP076706:c207120-206842 [Escherichia coli O170:H18]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T206379 NZ_CP102379:c1143448-1143341 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT206379 WP_000781370.1 NZ_CP076706:c206842-206558 [Escherichia coli O170:H18]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT206379 NZ_CP102379:1143495-1143561 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References