Detailed information of TA system TA206369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4594770..4595372
• Replicon: chromosome
• Accession: NZ_CP076704
• Organism: Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KQ221_RS22170
• Protein ID: WP_000897305.1
• Coordinates: 4595061..4595372 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KQ221_RS22165
• Protein ID: WP_000356397.1
• Coordinates: 4594770..4595060 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ221_RS22140 (4590696)	4590696..4591598	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KQ221_RS22145 (4591595)	4591595..4592230	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
KQ221_RS22150 (4592227)	4592227..4593156	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
KQ221_RS22155 (4593486)	4593486..4593728	-	243	WP_001086388.1	protein YiiF	-
KQ221_RS22160 (4593947)	4593947..4594165	-	219	WP_001295676.1	CopG family transcriptional regulator	-
KQ221_RS22165 (4594770)	4594770..4595060	-	291	WP_000356397.1	NadS family protein	Antitoxin
KQ221_RS22170 (4595061)	4595061..4595372	-	312	WP_000897305.1	hypothetical protein	Toxin
KQ221_RS22175 (4595601)	4595601..4596509	+	909	WP_001162704.1	alpha/beta hydrolase	-
KQ221_RS22180 (4596573)	4596573..4597514	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
KQ221_RS22185 (4597559)	4597559..4597996	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
KQ221_RS22190 (4597993)	4597993..4598865	-	873	WP_000920762.1	virulence factor BrkB family protein	-
KQ221_RS22195 (4598859)	4598859..4599458	-	600	WP_001295269.1	glucose-1-phosphatase	-
KQ221_RS22200 (4599557)	4599557..4600342	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T206369 WP_000897305.1 NZ_CP076704:c4595372-4595061 [Escherichia coli O8:H28]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T206369 NZ_CP102378:c3245091-3244777 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT206369 WP_000356397.1 NZ_CP076704:c4595060-4594770 [Escherichia coli O8:H28]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT206369 NZ_CP102378:c3244780-3244364 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7