Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 595931..596730 Replicon chromosome
Accession NZ_CP076704
Organism Escherichia coli O8:H28 strain BMH-17-0027

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag KQ221_RS02915 Protein ID WP_000347267.1
Coordinates 595931..596395 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KQ221_RS02920 Protein ID WP_001307405.1
Coordinates 596395..596730 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ221_RS02885 (590932) 590932..591366 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KQ221_RS02890 (591384) 591384..592262 - 879 WP_001442628.1 PTS N-acetylgalactosamine transporter subunit IID -
KQ221_RS02895 (592252) 592252..593031 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KQ221_RS02900 (593042) 593042..593515 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KQ221_RS02905 (593538) 593538..594818 - 1281 WP_000681936.1 tagatose-bisphosphate aldolase subunit KbaZ -
KQ221_RS02910 (595067) 595067..595876 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KQ221_RS02915 (595931) 595931..596395 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KQ221_RS02920 (596395) 596395..596730 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KQ221_RS02925 (596879) 596879..598450 - 1572 WP_097446335.1 galactarate dehydratase -
KQ221_RS02930 (598825) 598825..600159 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
KQ221_RS02935 (600175) 600175..600945 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 595931..607386 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T206347 WP_000347267.1 NZ_CP076704:c596395-595931 [Escherichia coli O8:H28]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T206347 NZ_CP102374:5064360-5064653 [Pseudomonas sp. T8]
ATGAACGAACCGCTTTTCGACTATGACCCCGCCACGGCCTTGCGCGACCCGCAAGCCATTGCCCTGTTCATGGCGGACGC
CTTCGAGACCGGCGATGCCGGCTACATCGCCAAAGCCCTGGGCGTGGTGGCGCGGGCCAAGGGCATGACCGAACTGGCCC
ATGAAACCGGGCTGTCCCGCGAGCAGCTGTACCGCTCGTTCAGTGACAAGGGCAACCCGACCCTGAAGACCCTGCTGGCG
GTGATGCGCGCCCTGGGGCTGGAGATGACGGCGCGGGTGCATGGGGCGTCTTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT206347 WP_001307405.1 NZ_CP076704:c596730-596395 [Escherichia coli O8:H28]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT206347 NZ_CP102374:5064064-5064363 [Pseudomonas sp. T8]
ATGATCGAGATCAAACAGACCGCGACCTTTATGGAGTGGGAAAACAGGCTCAAGGATTCCAGAGCCAGGGCGTTGATCGC
TGCGCGGATTTTTCGCCTGGCCAATGGCCTTGCCGGTGACGTGCCACCGGTGGGCGAGGGCGTCAGCGAGTTGCGCATCC
ATTACGGGCCGGGCTACCGAGTTTATTTCCAGCAGCTTGGTTCGCAACTTGTCATTTTGTTATGCGGCGGCGACAAGAGT
CGCCAGAGCCGCGATATCGAACTGGCCAAATACCTGGCCCGTCGTTGGAGCCGATCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References