Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3527797..3528596 Replicon chromosome
Accession NZ_CP076697
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KQ259_RS17195 Protein ID WP_000347251.1
Coordinates 3528132..3528596 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag KQ259_RS17190 Protein ID WP_001296435.1
Coordinates 3527797..3528132 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS17175 (3523582) 3523582..3524352 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
KQ259_RS17180 (3524368) 3524368..3525702 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KQ259_RS17185 (3526077) 3526077..3527648 + 1572 WP_001273763.1 galactarate dehydratase -
KQ259_RS17190 (3527797) 3527797..3528132 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KQ259_RS17195 (3528132) 3528132..3528596 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KQ259_RS17200 (3528651) 3528651..3529460 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KQ259_RS17205 (3529709) 3529709..3530989 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
KQ259_RS17210 (3531012) 3531012..3531485 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KQ259_RS17215 (3531496) 3531496..3532275 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KQ259_RS17220 (3532265) 3532265..3533143 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KQ259_RS17225 (3533161) 3533161..3533595 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T206329 WP_000347251.1 NZ_CP076697:3528132-3528596 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T206329 NZ_CP102358:2063143-2063355 [Pseudomonas zeae]
GTGCAAAGCAGGTTATTGATCAAGGAGCTGGAGGAGGCGGGTTGGACGCTGGACCGGATTACCGGCAGTCATCACATTTT
CAGGCACCGTTACAACCCTTACACGATTCCCGTTCCTCATCCGAAAAAGGATTTGCCGTTGGGGACAGTCAAAAGCATCA
GGAGACGTGCCGGGTTGTACAACCCGCCAGCCAGTTTCGAAGGAGATCCATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT206329 WP_001296435.1 NZ_CP076697:3527797-3528132 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT206329 NZ_CP102358:2063355-2063759 [Pseudomonas zeae]
ATGCAATATCCAATCTGTATCGAGTGGGGAGACGAGAACACTGCCATCGGTATTCAGATCCCCGATATTCCCGGCGCCGT
TACAGCGGGGGATAGCTTTGAAGATGCCTACAACGCGGCGGTTGAAGTCGCCCACATCATGCTGCAGGAGATTGCGGCGG
ACGGGGAGTCGATTCCTATGCCGACTTCGGCATCCGCTCATCGCAGTAATCCGGACTTTGCCGACATGGGTTGGGGCATG
CTCGAGCTGGATATTTCGCCTTATCTCGGCAAGACCGAAAAGGTCAACGTGACGTTGCCTGGCTATGTAATTCAGCGCAT
CGATCGTTATGTGCGCGAACACAACGTCAAAAGCCGTTCCTCCTTTCTGGCGGATGCGGCGATGGAGAAACTGGTTCGTT
ACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References