206328

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3486089..3486816 Replicon chromosome
Accession NZ_CP076697
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KQ259_RS16975 Protein ID WP_000550189.1
Coordinates 3486502..3486816 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KQ259_RS16970 Protein ID WP_000560269.1
Coordinates 3486089..3486505 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS16960 (3481449) 3481449..3483800 + 2352 WP_000695432.1 alpha-glucosidase -
KQ259_RS16965 (3484026) 3484026..3486044 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KQ259_RS16970 (3486089) 3486089..3486505 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KQ259_RS16975 (3486502) 3486502..3486816 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KQ259_RS16980 (3487101) 3487101..3488237 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KQ259_RS16985 (3488322) 3488322..3488825 + 504 WP_001295542.1 M48 family metallopeptidase -
KQ259_RS16990 (3488902) 3488902..3489594 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
KQ259_RS16995 (3489673) 3489673..3490659 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T206328 WP_000550189.1 NZ_CP076697:c3486816-3486502 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T206328 NZ_CP102358:c947094-946792 [Pseudomonas zeae]
ATGATCGCTTTTGAACGATCCCGCATATTTTCCGAATGGCTCGACTCCTTAAAAGACATCAAAGGGAAAGGCCGAATCAT
CTCCAGGATTAAAACAGCCGAACATGGCAACTTCGGCGATTGCGGCTTCGTCGGTGACACCGTTTATGAAATGCGTGTTC
ATTACGGCCCTGGTTACAGAATGTACTTCACACGTAAAGGCGAAGTGATTTATCTGCTGTTGATCGGGGGCGACAAATCG
ACACAAAAGAGAGATATCAAACGCGCCGTGCAAATGGCTCATAACATCGGAAATGAGGAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT206328 WP_000560269.1 NZ_CP076697:c3486505-3486089 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT206328 NZ_CP102358:c946788-946498 [Pseudomonas zeae]
ATGACCGAAGAATTCGCCCGTTTTGACGCCGTCGAGTACCTCAAGACTCCCGAGGATATGGCGGCATATCTGGATGCCTG
TTTTGACGACGATGAAGGCGATGGCGTTCTGATTCGTGCAGCACTCAATGACATCGCCCGAGCTCAAGGCATGACACAAG
TGGCGCGGGATGCAGGTTTGGGTCGCGAAAGTCTTTATAAAGCGCTCAGTAGCACTGGCAATCCCGAATTCGCAACTATC
ATGAAAGTCATGAAGGCTCTGGGTTTGCGATTGCATGCCGTAACACTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References