Detailed information of TA system TA206324

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2236634..2237136
• Replicon: chromosome
• Accession: NZ_CP076697
• Organism: Escherichia coli strain S10EC

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: KQ259_RS11280
• Protein ID: WP_000767819.1
• Coordinates: 2236634..2236888 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: KQ259_RS11285
• Protein ID: WP_001259253.1
• Coordinates: 2236885..2237136 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ259_RS11255 (2232452)	2232452..2232910	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
KQ259_RS11260 (2233127)	2233127..2234485	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
KQ259_RS11265 (2234475)	2234475..2234537	-	63	WP_010723108.1	membrane protein YoeI	-
KQ259_RS11270 (2234752)	2234752..2235681	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KQ259_RS11275 (2235727)	2235727..2236551	-	825	WP_000754737.1	SDR family oxidoreductase	-
KQ259_RS11280 (2236634)	2236634..2236888	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KQ259_RS11285 (2236885)	2236885..2237136	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KQ259_RS11290 (2237419)	2237419..2237469	+	51	WP_001364200.1	his operon leader peptide	-
KQ259_RS11295 (2237615)	2237615..2238514	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KQ259_RS11300 (2238520)	2238520..2239824	+	1305	WP_001296211.1	histidinol dehydrogenase	-
KQ259_RS11305 (2239821)	2239821..2240891	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KQ259_RS11310 (2240891)	2240891..2241958	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2232452..2232910	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T206324 WP_000767819.1 NZ_CP076697:c2236888-2236634 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T206324 NZ_CP102355:c1409316-1409128 [Paracoccus sp. SMMA_5_TC]
ATGCAAATCGAACGAAACAGCCGGCAAATCCTACAGGTCTTGAAGGCAGCAGGGTTCGAGGAGGTCTCAAAACGGGGGTC
GCACCTCAAGCTGCGAAAAGGCGAAAGGACCGTGATCGTGCCACACCCGAAGAAAGACCTTCCCTTGGGAACTGCCCGCA
ACATCTACCAACAAGCCGGGCTCTTGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT206324 WP_001259253.1 NZ_CP076697:c2237136-2236885 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT206324 NZ_CP102355:c1409083-1408688 [Paracoccus sp. SMMA_5_TC]
ATGAAACAATACTCGGCAATCATCCACCACGATCCGGGGGCGGCTTTCGGTCTGACCTTCCCCGATCTTCCGGGATGCTT
TGCGGCTGCCGATTGCTGGGACGATGTCCCGAAGGCTGCATCCGAGGCCCTTAACCTGTGGTTCGAGGATCAGCCCGAGG
TTCCACCGTCACCCCTGGACGCCCTGCTCAAGCGACAGGATGTCGCCCAGGCCCTCGCAGAAGGCGCCACTCTGATCACG
ATCCCCTATATTCCCGCTGATGGGACATTGGAACGCGTGAACATCTCGATCGAGCGCGGCTTGCTTCGCGCTGTCGACGC
GGCTGCAAAGGAACGCGGCATGACCCGTTCGTCGTTTCTTGCCTCGGCAGCGCGCCATGAACTCACTGGCGCCTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3