206309

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 535735..536414 Replicon chromosome
Accession NZ_CP076697
Organism Escherichia coli strain S10EC

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag KQ259_RS02525 Protein ID WP_000057523.1
Coordinates 535735..536037 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag KQ259_RS02530 Protein ID WP_000806442.1
Coordinates 536073..536414 (+) Length 114 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ259_RS02500 (531111) 531111..532763 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
KQ259_RS02505 (532801) 532801..533304 - 504 WP_000667000.1 hypothetical protein -
KQ259_RS02510 (533301) 533301..534101 - 801 WP_000439798.1 hypothetical protein -
KQ259_RS02515 (534125) 534125..534604 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
KQ259_RS02520 (534808) 534808..535602 - 795 WP_000365147.1 TraB/GumN family protein -
KQ259_RS02525 (535735) 535735..536037 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KQ259_RS02530 (536073) 536073..536414 + 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
KQ259_RS02535 (536472) 536472..538976 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
KQ259_RS02540 (539238) 539238..540170 + 933 WP_000883041.1 glutaminase A -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(9-98)

Antitoxin

(1-100)


Sequences


Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T206309 WP_000057523.1 NZ_CP076697:535735-536037 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T206309 NZ_CP102346:1370070-1370462 [Mycolicibacterium smegmatis]
GTGACCGAGTACCTCGACCGCGAGGACGTGCTCACCGCGGGATCGATCGCTTTTGGCGGCGAGCTGAAGGTGCGGGACTA
CGGACTGCTCGACGCTGCGGTTGCCCGCCCGCAGGCCACGGTGTATGGCGTGGATGCGTACCCGCGGCTGTGGGACAAGG
CCGCTGCGTTGTTGCAGTCCCTGGCGCGGAACCATGCGCTGGTCGACGGTAACAAGCGCACTGCGTGGGCTGCGGCATGG
ACATTTCTGCACATCAACGGTGTACAGCTGGCGGCTGATTTCGATGTCGATCGCGCGGAGGACCTCATGAACGAGGTGGC
CACCCGTGACTGCGACCTGGATTCCATCGCCGCCGAGCTGGCCGGTTTCGCCGCTGCTGCGCAGACGGGGTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT206309 WP_000806442.1 NZ_CP076697:536073-536414 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT206309 NZ_CP102346:1369876-1370073 [Mycolicibacterium smegmatis]
ATGCCGTCGCTGAACATCGACTTCGACGAAGCCGAGATGGAACAGATCCGCGCTGCTGCCCGGGCGGATGACCTGTCGCT
GAAGAAGTTCGCGCACGCCGCCGTGATGGAGCGTGCCAGCGCGCACAAGCGCAGGGTCGCCGAGGCGGCGCGTCTGGTCG
CTGAGCGTTCGGCTGAGCTCAACCGCCGCTTGGCGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1PK60


Antitoxin

Source ID Structure
AlphaFold DB S1QAY3

References