Detailed information of TA system TA206296

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 26488..26719
• Replicon: plasmid pS103EC_A
• Accession: NZ_CP076694
• Organism: Escherichia coli strain S103EC

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: KQ261_RS25490
• Protein ID: WP_023144756.1
• Coordinates: 26488..26622 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	26668..26719 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ261_RS25450 (22355)	22355..22981	+	627	WP_252397316.1	EAL domain-containing protein	-
KQ261_RS25455 (23347)	23347..23632	-	286	Protein_27	type II toxin-antitoxin system RelE/ParE family toxin	-
KQ261_RS25460 (23622)	23622..23876	-	255	WP_000108831.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KQ261_RS25465 (23932)	23932..24072	-	141	WP_165395303.1	hypothetical protein	-
KQ261_RS25470 (24776)	24776..25633	-	858	WP_032265352.1	incFII family plasmid replication initiator RepA	-
KQ261_RS25475 (25626)	25626..25700	-	75	WP_031943482.1	RepA leader peptide Tap	-
KQ261_RS25480 (25700)	25700..25831	-	132	Protein_32	protein CopA/IncA	-
KQ261_RS25485 (25937)	25937..26191	-	255	WP_000083850.1	replication regulatory protein RepA	-
KQ261_RS25490 (26488)	26488..26622	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (26668)	26668..26719	+	52	NuclAT_1	-	Antitoxin
- (26668)	26668..26719	+	52	NuclAT_1	-	Antitoxin
- (26668)	26668..26719	+	52	NuclAT_1	-	Antitoxin
- (26668)	26668..26719	+	52	NuclAT_1	-	Antitoxin
KQ261_RS25495 (26686)	26686..26972	-	287	Protein_35	DUF2726 domain-containing protein	-
KQ261_RS25500 (27485)	27485..27697	-	213	WP_013023861.1	hypothetical protein	-
KQ261_RS25505 (27828)	27828..28388	-	561	WP_000139341.1	fertility inhibition protein FinO	-
KQ261_RS25510 (28443)	28443..29189	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..146805	146805

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T206296 WP_023144756.1 NZ_CP076694:c26622-26488 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T206296 NZ_CP102343:3517540-3518118 [Mycolicibacterium smegmatis]
GTGAAACGACTCGATCTCGTCGTTGGTCCCAACGGCGCGGGCAAGAGCACTTTCGTCGAACTCACATTGGCCCCGCTGCT
GACGGGCAGTGCGTTCGTCAACGCCGACGAGATCGCCAAGCGTCGCTGGCCGCACGCCCCGGCCGAGCACTCCTACGAGG
CCGCACGCATCGCCTCCGACACCCGACTCAAGCTGATCGAACTCGGTGAATCGTTCATCGCCGAAACGGTGTTCTCACAT
CCCTCGAAACTTCAGCTCATCGACACCGCGCAGGCGGCTGGATACACCGTGGTAATGCACGTGGTGATGATCCCCGAGGA
TCTCGCCGTCCTACGCGTGCAGCACCGGGTACGAGCCGGTGGTCACCATGTGCCCGAGGAGAAGATCCGCGAACGGCACC
AACGACTCTGGCCCCTCGTCGCGCTGGCGGCCGCCCGCGCCGACATCTCGACGTTCTATGACAACAGCGCCGTCAGGGGT
CCGCGCATCGTTGCCCAACTCGCCGGCGGATTTGCTGTGGGCCGGCCCGCCTGGCCAATGTGGGCACCCCAGGTGCTCAC
CTCGCGGTGGCCCGAATAG

Antitoxin

Nucleotide

• Length: 52 bp

>AT206296 NZ_CP076694:26668-26719 [Escherichia coli]
TCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin