Detailed information of TA system TA206289

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4407187..4407789
• Replicon: chromosome
• Accession: NZ_CP076693
• Organism: Escherichia coli strain S103EC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: KQ261_RS21580
• Protein ID: WP_000897302.1
• Coordinates: 4407187..4407498 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KQ261_RS21585
• Protein ID: WP_000356397.1
• Coordinates: 4407499..4407789 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ261_RS21555 (4403101)	4403101..4403700	+	600	WP_001296610.1	glucose-1-phosphatase	-
KQ261_RS21560 (4403694)	4403694..4404566	+	873	WP_000920762.1	virulence factor BrkB family protein	-
KQ261_RS21565 (4404563)	4404563..4405000	+	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
KQ261_RS21570 (4405045)	4405045..4405986	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KQ261_RS21575 (4406050)	4406050..4406958	-	909	WP_001385591.1	alpha/beta hydrolase	-
KQ261_RS21580 (4407187)	4407187..4407498	+	312	WP_000897302.1	hypothetical protein	Toxin
KQ261_RS21585 (4407499)	4407499..4407789	+	291	WP_000356397.1	NadS family protein	Antitoxin
KQ261_RS21590 (4408148)	4408148..4408426	+	279	WP_001296612.1	hypothetical protein	-
KQ261_RS21595 (4408823)	4408823..4409041	+	219	WP_001251293.1	CopG family transcriptional regulator	-
KQ261_RS26470 (4409226)	4409226..4409966	-	741	WP_000608806.1	hypothetical protein	-
KQ261_RS21605 (4409991)	4409991..4410839	-	849	WP_001038650.1	hypothetical protein	-
KQ261_RS21610 (4411129)	4411129..4411371	+	243	WP_001068514.1	CopG family transcriptional regulator	-
KQ261_RS21615 (4411553)	4411553..4412482	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T206289 WP_000897302.1 NZ_CP076693:4407187-4407498 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T206289 NZ_CP102342:1370070-1370462 [Mycolicibacterium smegmatis]
GTGACCGAGTACCTCGACCGCGAGGACGTGCTCACCGCGGGATCGATCGCTTTTGGCGGCGAGCTGAAGGTGCGGGACTA
CGGACTGCTCGACGCTGCGGTTGCCCGCCCGCAGGCCACGGTGTATGGCGTGGATGCGTACCCGCGGCTGTGGGACAAGG
CCGCTGCGTTGTTGCAGTCCCTGGCGCGGAACCATGCGCTGGTCGACGGTAACAAGCGCACTGCGTGGGCTGCGGCATGG
ACATTTCTGCACATCAACGGTGTACAGCTGGCGGCTGATTTCGATGTCGATCGCGCGGAGGACCTCATGAACGAGGTGGC
CACCCGTGACTGCGACCTGGATTCCATCGCCGCCGAGCTGGCCGGTTTCGCCGCTGCTGCGCAGACGGGGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT206289 WP_000356397.1 NZ_CP076693:4407499-4407789 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT206289 NZ_CP102342:1369876-1370073 [Mycolicibacterium smegmatis]
ATGCCGTCGCTGAACATCGACTTCGACGAAGCCGAGATGGAACAGATCCGCGCTGCTGCCCGGGCGGATGACCTGTCGCT
GAAGAAGTTCGCGCACGCCGCCGTGATGGAGCGTGCCAGCGCGCACAAGCGCAGGGTCGCCGAGGCGGCGCGTCTGGTCG
CTGAGCGTTCGGCTGAGCTCAACCGCCGCTTGGCGTGA

Structures

Toxin

• AlphaFold DB: S1P416