Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3596274..3597073 Replicon chromosome
Accession NZ_CP076693
Organism Escherichia coli strain S103EC

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KQ261_RS17625 Protein ID WP_000347251.1
Coordinates 3596609..3597073 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag KQ261_RS17620 Protein ID WP_001296435.1
Coordinates 3596274..3596609 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ261_RS17605 (3592059) 3592059..3592829 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
KQ261_RS17610 (3592845) 3592845..3594179 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KQ261_RS17615 (3594554) 3594554..3596125 + 1572 WP_001273763.1 galactarate dehydratase -
KQ261_RS17620 (3596274) 3596274..3596609 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KQ261_RS17625 (3596609) 3596609..3597073 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KQ261_RS17630 (3597128) 3597128..3597937 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KQ261_RS17635 (3598186) 3598186..3599466 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
KQ261_RS17640 (3599489) 3599489..3599962 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KQ261_RS17645 (3599973) 3599973..3600752 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KQ261_RS17650 (3600742) 3600742..3601620 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KQ261_RS17655 (3601638) 3601638..3602072 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T206287 WP_000347251.1 NZ_CP076693:3596609-3597073 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T206287 NZ_CP102330:2146572-2147153 [Burkholderia cepacia]
ATGAACCCCGATACGATCCACGCGCGGCTCGCGTTCCTGCGCGAAGCCGAACGCCTGAAGGATGTGCTGCGCAGCGGCCA
CACGTCGGCCGGGCGCGCGGAAAGCACCGCCGAGCATAGCTGGCGGCTGTGCCTGATGGCACTCGTATTCGCCGATGCGC
TGCCCGGCATCGACACGCTGAAGCTGCTGAAGCTGTGTGTCGTCCACGATCTCGGCGAGGTGCTGCACGGCGACATCCCC
GCGATCGAGCAGGCCGCGCACCCCGACAAGAGCGCGCACGAACGCGACGACCTGCTGACGCTGACCGCGCCGCTCGATCC
GGCGCAGCGCGACGAGATCGTCGCACTGTGGGATGAATACGAAGCGGCCGCGTCGCCGGAAGCGCGCGCGGCGAAAGCGT
TCGACAAGCTGGAGACGATCCTGCAGCACACCCAGGGCCGCAATCCGCCCGGTTTCGACTACGCGTTCAATCTCGGCTAC
GGCCGCCGCTATACCGACGCCGCACCGCTGTTCAGCGCGATCCGCGACATCGTCGACGCCGACACGCAACGCCGGATCGA
CGCGAGCGGGCAGCACGCGTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT206287 WP_001296435.1 NZ_CP076693:3596274-3596609 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT206287 NZ_CP102330:2145932-2146489 [Burkholderia cepacia]
ATGTCTGCCTCACCGCTACACACGCCGCCACTCGGCCCGCAACTCAAGCGCTGGCGTGCGCTGCACCGCGTGAAACAGAG
CCACGCGGCGGAACTGTTCGGCGTGGCGCAGTCGACGATCTCACGCTGGGAAGCCGGCCTGCAACAGATGTCGCCCGGCG
AACGCGCGACGGCCGAACGGCTGCTCGCCGCGCGCCTCGATTCCGCGGGCGATCACGCGCTTGCGCGGCTCGTCGCGGGC
AGCGCGGGCCGCATGCATCTCGTGTGCGACCTCACGCACCGGCTGCTCGCCTGTTCGCCGGCGCGTGCAGCCGAATTCTC
GCAACCGCTGTCGATGCTGCTCGGCACGTCGCTGTGGCGCTATGCGACGCCGGAAATCGTCCGCATGGAAACCGCGCTCG
ACACGCTCGGCTGGCATGATCGCGCGGGACCGCCGAGCGTCGAATTCGACACCGGCGCGAATGCGTCGCGCGTCGTGCCG
ATCCGCGGCAGCCTGTGCCGGTGGACACGGATGACGTTGTCGGACGGGTCGGCCGCGCGGCTCGTGGAAACGCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References