Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3554566..3555293 Replicon chromosome
Accession NZ_CP076693
Organism Escherichia coli strain S103EC

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KQ261_RS17405 Protein ID WP_000550189.1
Coordinates 3554979..3555293 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KQ261_RS17400 Protein ID WP_000560269.1
Coordinates 3554566..3554982 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ261_RS17390 (3549926) 3549926..3552277 + 2352 WP_000695432.1 alpha-glucosidase -
KQ261_RS17395 (3552503) 3552503..3554521 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KQ261_RS17400 (3554566) 3554566..3554982 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KQ261_RS17405 (3554979) 3554979..3555293 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KQ261_RS17410 (3555578) 3555578..3556714 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KQ261_RS17415 (3556799) 3556799..3557302 + 504 WP_001295542.1 M48 family metallopeptidase -
KQ261_RS17420 (3557379) 3557379..3558071 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
KQ261_RS17425 (3558150) 3558150..3559136 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T206286 WP_000550189.1 NZ_CP076693:c3555293-3554979 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T206286 NZ_CP102330:c1990270-1989851 [Burkholderia cepacia]
ATGATCCTTGTTGATACGAATGTCATTTCCGAACCGCTACGACGCGAGCCCAACGCGGCCGTGATCGAGTGGCTCGATGC
CCAGAATGTCGAAACCTTGTTTCTGGCTGCGATCAGTCTCGCGGAAATACGATTGGGCGTGGCTGTATTGCCGGAAGGAC
GCCGGCGAGAGTGGCTGCATCAGAGCATCGAGCAGCGCGTGCTGCCATTGTTTCGCGGACGAATCCTGCCATTCGACGAT
GCTGCGAGCAAAGCGTATGCGAGCCTTCGCGCACGCGCTCGTGCCGCAGGTGTCGCAATTGCACCATCCGATGGTTTTAT
CGCCGGCACGGCCGAAGCGAACGGCCTGATCGTCGCCACGCGCGATGTCACGCCGTTCGAGGCAATGGGAATTCGCGTAA
TCGACCCGTGGGCGCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT206286 WP_000560269.1 NZ_CP076693:c3554982-3554566 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT206286 NZ_CP102330:c1990506-1990267 [Burkholderia cepacia]
ATGCCGGTGATCACCGTCCGAAATCTGTCCGATGAAGTGCATCGAGCACTTCGCATTCGCGCGGCCCAACATGGGCGCAG
CACCGAAGCCGAGGTGCGCGACATTCTCGAACAGGCCGTTCTTCCGGGCGGGCGGCTCAAGCTGGGAACGATGCTGGCGG
CAATCGGGCAGGAGGCCGGCGGATTCGATTTCGACGCCCAGCGCGACAAGACGCCTGCCGATCCGATGAGCTTCGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References