Detailed information of TA system TA206283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2299771..2300273
• Replicon: chromosome
• Accession: NZ_CP076693
• Organism: Escherichia coli strain S103EC

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: KQ261_RS11675
• Protein ID: WP_000767819.1
• Coordinates: 2299771..2300025 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: KQ261_RS11680
• Protein ID: WP_001259253.1
• Coordinates: 2300022..2300273 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ261_RS11650 (2295589)	2295589..2296047	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
KQ261_RS11655 (2296264)	2296264..2297622	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
KQ261_RS11660 (2297612)	2297612..2297674	-	63	WP_010723108.1	membrane protein YoeI	-
KQ261_RS11665 (2297889)	2297889..2298818	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KQ261_RS11670 (2298864)	2298864..2299688	-	825	WP_000754737.1	SDR family oxidoreductase	-
KQ261_RS11675 (2299771)	2299771..2300025	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KQ261_RS11680 (2300022)	2300022..2300273	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KQ261_RS11685 (2300556)	2300556..2300606	+	51	WP_001364200.1	his operon leader peptide	-
KQ261_RS11690 (2300752)	2300752..2301651	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KQ261_RS11695 (2301657)	2301657..2302961	+	1305	WP_001296211.1	histidinol dehydrogenase	-
KQ261_RS11700 (2302958)	2302958..2304028	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KQ261_RS11705 (2304028)	2304028..2305095	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2295589..2296047	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T206283 WP_000767819.1 NZ_CP076693:c2300025-2299771 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T206283 NZ_CP102330:c791856-791491 [Burkholderia cepacia]
ATGGTAAAAGCTCTCTTCGATACCAACATCCTGATCGACTATCTGGGCGGCGTGGAATCCGCTCGTGCGGAACTGGGCCG
CTACGACTATCGGGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGCACGTCCGCACAGGACGAGGCACCCATCC
GCGCGTGGCTGTCGTCGTTCGATGTCATCGCACTGGACAGCCCGGTTGCGAATCGTGCCGTGACAATCCGCAAGGAACGC
CGCATACGCCTTCCCGATGCAATCGTCTGGGCATCGGCACAAGTGAACGGTCTACTGCTCGTGTCGCGCAACACGAAGGA
TTTCCCTGCCACCGAACCCGGCGTTCGCGTGCCCTACAAGCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT206283 WP_001259253.1 NZ_CP076693:c2300273-2300022 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT206283 NZ_CP102330:c792068-791850 [Burkholderia cepacia]
ATGAGCCGAATTCTGGTCGATCTGTCGGACGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACATCGTCCCCG
CGCCGCAATCATCCGCGACGCGATCGATGCCTATATTGCCTTGCACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCAAGGTCGACGGGCTCACCTATCAGGAAGAACTGCGCTCGGAATGGTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3