Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3611462..3612261 Replicon chromosome
Accession NZ_CP076689
Organism Escherichia coli strain S21EC

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KQ260_RS17855 Protein ID WP_000347251.1
Coordinates 3611797..3612261 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag KQ260_RS17850 Protein ID WP_001296435.1
Coordinates 3611462..3611797 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ260_RS17835 (3607247) 3607247..3608017 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
KQ260_RS17840 (3608033) 3608033..3609367 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KQ260_RS17845 (3609742) 3609742..3611313 + 1572 WP_001273763.1 galactarate dehydratase -
KQ260_RS17850 (3611462) 3611462..3611797 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KQ260_RS17855 (3611797) 3611797..3612261 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KQ260_RS17860 (3612316) 3612316..3613125 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KQ260_RS17865 (3613374) 3613374..3614654 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
KQ260_RS17870 (3614677) 3614677..3615150 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KQ260_RS17875 (3615161) 3615161..3615940 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KQ260_RS17880 (3615930) 3615930..3616808 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KQ260_RS17885 (3616826) 3616826..3617260 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T206256 WP_000347251.1 NZ_CP076689:3611797-3612261 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T206256 NZ_CP102247:c3157922-3157401 [Enterobacter asburiae]
GTGGCAAATCTCACGATCGAAATGTTATCTGAGGGAACCGATTACGACTTCGAGGATTTTGATTGTGGAGAACCCTCGCT
AAATGCCTTCCTTGCAGAACACCTGGTACGTCAGCACAATGGGCGTATTTTACGAGCCTACCTGCTTAAAGAGCGCGATC
GTCCGCGCGTCCTGGGTTATTACACGTTATCGGGAAGCTGCTTTGAAAGAGCGATGCTTCCTTCTAAGACGCAGCAGCGC
CGGATCCCTTATATCAACGTACCCAGCGTGACGCTGGGACGTCTGGCTGTAGATAAAACACTACAGGGAAACGAGTGGGG
CACAACGCTCGTTGCGCATGCTATGCGCGTTGTCTACCTGGCATCACAGGCTGTGGGGGTACACGGAATATTTGTTGATG
CCCTTAACGAGCAGGCCAAACAATTTTACCTGAAGCAGGGGTTTATCCCTCTGACAGGTGAAAACAGCCATTCTTTGTTC
TTCCCTACAAAATCTATCGAACGGCTGTTTGAGCAAGAGTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT206256 WP_001296435.1 NZ_CP076689:3611462-3611797 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT206256 NZ_CP102247:c3158198-3157929 [Enterobacter asburiae]
ATGCCAGCACTTAAAAAACAGCGTATCGATCTTCGCTTAACGGACGAAGACAAAACAATGATTGAAGAAGCGGCGGCGAT
GACGAACCAGACCATCACCCAGTTTATGGTGAACAGTGCGTCAGAGCGTGCTGCAGAGGTCATTGAGCAGCACCGTCGAC
TCGTATTAAATGAAGCATCATGGAATGCGGTGATGGATGCCATTGACAATCCGCCTGAGCCAATCGAACGCTTGAAACGA
GCAGCAAAACGTCTTCGAAACATGGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References