Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3571007..3571734 Replicon chromosome
Accession NZ_CP076689
Organism Escherichia coli strain S21EC

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KQ260_RS17645 Protein ID WP_000550189.1
Coordinates 3571420..3571734 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KQ260_RS17640 Protein ID WP_000560269.1
Coordinates 3571007..3571423 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ260_RS17630 (3566367) 3566367..3568718 + 2352 WP_001531994.1 alpha-glucosidase -
KQ260_RS17635 (3568944) 3568944..3570962 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KQ260_RS17640 (3571007) 3571007..3571423 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KQ260_RS17645 (3571420) 3571420..3571734 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KQ260_RS17650 (3572019) 3572019..3573155 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KQ260_RS17655 (3573240) 3573240..3573743 + 504 WP_001295542.1 M48 family metallopeptidase -
KQ260_RS17660 (3573820) 3573820..3574512 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
KQ260_RS17665 (3574591) 3574591..3575577 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T206255 WP_000550189.1 NZ_CP076689:c3571734-3571420 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T206255 NZ_CP102247:1988694-1988797 [Enterobacter asburiae]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT206255 WP_000560269.1 NZ_CP076689:c3571423-3571007 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT206255 NZ_CP102247:c1988803-1988659 [Enterobacter asburiae]
ACAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCCTTTTAAGAATAGATGACGACGCCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References