Detailed information of TA system TA206252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2336208..2336710
• Replicon: chromosome
• Accession: NZ_CP076689
• Organism: Escherichia coli strain S21EC

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: KQ260_RS12005
• Protein ID: WP_000767819.1
• Coordinates: 2336208..2336462 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: KQ260_RS12010
• Protein ID: WP_001259253.1
• Coordinates: 2336459..2336710 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ260_RS11980 (2332026)	2332026..2332484	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
KQ260_RS11985 (2332701)	2332701..2334059	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
KQ260_RS11990 (2334049)	2334049..2334111	-	63	WP_010723108.1	membrane protein YoeI	-
KQ260_RS11995 (2334326)	2334326..2335255	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KQ260_RS12000 (2335301)	2335301..2336125	-	825	WP_000754737.1	SDR family oxidoreductase	-
KQ260_RS12005 (2336208)	2336208..2336462	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KQ260_RS12010 (2336459)	2336459..2336710	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KQ260_RS12015 (2336993)	2336993..2337043	+	51	WP_001364200.1	his operon leader peptide	-
KQ260_RS12020 (2337189)	2337189..2338088	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KQ260_RS12025 (2338094)	2338094..2339398	+	1305	WP_001296211.1	histidinol dehydrogenase	-
KQ260_RS12030 (2339395)	2339395..2340465	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KQ260_RS12035 (2340465)	2340465..2341532	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2332026..2332484	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T206252 WP_000767819.1 NZ_CP076689:c2336462-2336208 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T206252 NZ_CP102246:c4940234-4940049 [Enterobacter cloacae complex sp. R_G8]
GTGAAAAGTGCGGATGTCATTACTGTTCTGGTCAGGCATGGCTGGAAATGTGTAAGAACGAAAGGAAGCCATCATCAGTT
TCGCCACCCAGTACATGCGGGGTTGGTAACCGTTCCACATCCCAAAAAGGACATAAAACCCGGCACCCTCGCGCAAATCT
GGCGGCAGGCGGGAATCAAACACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT206252 WP_001259253.1 NZ_CP076689:c2336710-2336459 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT206252 NZ_CP102246:c4940034-4939630 [Enterobacter cloacae complex sp. R_G8]
ATGTTTTATCCGGCTTACATTCATTCTGATCCCGACGGTAGCGCCAGCGGTTTTTTCCCTGACGTTCCTGGTTGTTTTTT
TGCCGGCAATTCTCTGGATGACGCTTTTCAGGATGCACGTGATGCACTGACAGCTCACTTCGAAGCGCTATTTGAAATGG
ATGAAGAGTTACCTCTCCCTGGCAATGTTGAAGCACATCTTGAAGCCACACCGGGTGATTTTATCGGCGGACAATGGTTG
CTTGTAGATATCAATATGAAGCAATTCGACGGTAAGGCCGAACGTATTAATATCACCCTGCCTCGTCGATTGCTGGTGAA
AATTGATTCTTTTGTGAGTGAACATCCGCAGTTTAGTAACCGAAGCGCTTTTCTGGCTGAGGCAGCGCGTCGCGTGCTGC
CTTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3