Detailed information of TA system TA206229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4401678..4402280
• Replicon: chromosome
• Accession: NZ_CP076687
• Organism: Escherichia coli strain S22EC

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: KQ258_RS21515
• Protein ID: WP_000897302.1
• Coordinates: 4401678..4401989 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KQ258_RS21520
• Protein ID: WP_000356397.1
• Coordinates: 4401990..4402280 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ258_RS21490 (4397592)	4397592..4398191	+	600	WP_001296610.1	glucose-1-phosphatase	-
KQ258_RS21495 (4398185)	4398185..4399057	+	873	WP_000920762.1	virulence factor BrkB family protein	-
KQ258_RS21500 (4399054)	4399054..4399491	+	438	WP_000560981.1	D-aminoacyl-tRNA deacylase	-
KQ258_RS21505 (4399536)	4399536..4400477	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
KQ258_RS21510 (4400541)	4400541..4401449	-	909	WP_001385591.1	alpha/beta hydrolase	-
KQ258_RS21515 (4401678)	4401678..4401989	+	312	WP_000897302.1	hypothetical protein	Toxin
KQ258_RS21520 (4401990)	4401990..4402280	+	291	WP_000356397.1	NadS family protein	Antitoxin
KQ258_RS21525 (4402639)	4402639..4402917	+	279	WP_001296612.1	hypothetical protein	-
KQ258_RS21530 (4403314)	4403314..4403532	+	219	WP_001251293.1	CopG family transcriptional regulator	-
KQ258_RS25600 (4403717)	4403717..4404457	-	741	WP_000608806.1	hypothetical protein	-
KQ258_RS21540 (4404482)	4404482..4405330	-	849	WP_001038650.1	hypothetical protein	-
KQ258_RS21545 (4405620)	4405620..4405862	+	243	WP_001068514.1	CopG family transcriptional regulator	-
KQ258_RS21550 (4406044)	4406044..4406973	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T206229 WP_000897302.1 NZ_CP076687:4401678-4401989 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T206229 NZ_CP102244:1961987-1962238 [Escherichia coli O7:H15]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT206229 WP_000356397.1 NZ_CP076687:4401990-4402280 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT206229 NZ_CP102244:1962235-1962489 [Escherichia coli O7:H15]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CATTGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: S1P416