Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3592347..3593146 Replicon chromosome
Accession NZ_CP076687
Organism Escherichia coli strain S22EC

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KQ258_RS17575 Protein ID WP_000347251.1
Coordinates 3592682..3593146 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag KQ258_RS17570 Protein ID WP_001296435.1
Coordinates 3592347..3592682 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ258_RS17555 (3588132) 3588132..3588902 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
KQ258_RS17560 (3588918) 3588918..3590252 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
KQ258_RS17565 (3590627) 3590627..3592198 + 1572 WP_001273763.1 galactarate dehydratase -
KQ258_RS17570 (3592347) 3592347..3592682 + 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KQ258_RS17575 (3592682) 3592682..3593146 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KQ258_RS17580 (3593201) 3593201..3594010 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KQ258_RS17585 (3594259) 3594259..3595539 + 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
KQ258_RS17590 (3595562) 3595562..3596035 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KQ258_RS17595 (3596046) 3596046..3596825 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KQ258_RS17600 (3596815) 3596815..3597693 + 879 WP_216696511.1 PTS N-acetylgalactosamine transporter subunit IID -
KQ258_RS17605 (3597711) 3597711..3598145 + 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T206227 WP_000347251.1 NZ_CP076687:3592682-3593146 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T206227 NZ_CP102244:970254-970661 [Escherichia coli O7:H15]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT206227 WP_001296435.1 NZ_CP076687:3592347-3592682 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT206227 NZ_CP102244:970007-970273 [Escherichia coli O7:H15]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCCGATC
TGTTTAACTGGCTGATGAACCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References