Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3551892..3552619 Replicon chromosome
Accession NZ_CP076687
Organism Escherichia coli strain S22EC

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KQ258_RS17365 Protein ID WP_000550189.1
Coordinates 3552305..3552619 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KQ258_RS17360 Protein ID WP_000560269.1
Coordinates 3551892..3552308 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KQ258_RS17350 (3547252) 3547252..3549603 + 2352 WP_001531994.1 alpha-glucosidase -
KQ258_RS17355 (3549829) 3549829..3551847 + 2019 WP_000121413.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KQ258_RS17360 (3551892) 3551892..3552308 - 417 WP_000560269.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KQ258_RS17365 (3552305) 3552305..3552619 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KQ258_RS17370 (3552904) 3552904..3554040 - 1137 WP_000018703.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KQ258_RS17375 (3554125) 3554125..3554628 + 504 WP_001295542.1 M48 family metallopeptidase -
KQ258_RS17380 (3554705) 3554705..3555397 + 693 WP_000942551.1 vancomycin high temperature exclusion protein -
KQ258_RS17385 (3555476) 3555476..3556462 + 987 WP_001385498.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T206226 WP_000550189.1 NZ_CP076687:c3552619-3552305 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T206226 NZ_CP102244:c845713-845339 [Escherichia coli O7:H15]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGTTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCGC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACTCCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGAACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15023.47 Da        Isoelectric Point: 4.4547

>AT206226 WP_000560269.1 NZ_CP076687:c3552308-3551892 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFVEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT206226 NZ_CP102244:c846134-845760 [Escherichia coli O7:H15]
GTGTCAGACACACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References