Detailed information of TA system TA206223

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 2249598..2250100
• Replicon: chromosome
• Accession: NZ_CP076687
• Organism: Escherichia coli strain S22EC

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: KQ258_RS11370
• Protein ID: WP_000767819.1
• Coordinates: 2249598..2249852 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: KQ258_RS11375
• Protein ID: WP_001259253.1
• Coordinates: 2249849..2250100 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ258_RS11345 (2245416)	2245416..2245874	-	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-
KQ258_RS11350 (2246091)	2246091..2247449	-	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
KQ258_RS11355 (2247439)	2247439..2247501	-	63	WP_010723108.1	membrane protein YoeI	-
KQ258_RS11360 (2247716)	2247716..2248645	-	930	WP_000803366.1	LysR substrate-binding domain-containing protein	-
KQ258_RS11365 (2248691)	2248691..2249515	-	825	WP_000754737.1	SDR family oxidoreductase	-
KQ258_RS11370 (2249598)	2249598..2249852	-	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KQ258_RS11375 (2249849)	2249849..2250100	-	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KQ258_RS11380 (2250383)	2250383..2250433	+	51	WP_001364200.1	his operon leader peptide	-
KQ258_RS11385 (2250579)	2250579..2251478	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KQ258_RS11390 (2251484)	2251484..2252788	+	1305	WP_001296211.1	histidinol dehydrogenase	-
KQ258_RS11395 (2252785)	2252785..2253855	+	1071	WP_000108967.1	histidinol-phosphate transaminase	-
KQ258_RS11400 (2253855)	2253855..2254922	+	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2245416..2245874	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T206223 WP_000767819.1 NZ_CP076687:c2249852-2249598 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T206223 NZ_CP102244:159069-159254 [Escherichia coli O7:H15]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT206223 WP_001259253.1 NZ_CP076687:c2250100-2249849 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT206223 NZ_CP102244:159271-159681 [Escherichia coli O7:H15]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTATGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3