Detailed information of TA system TA206204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 908512..909117
• Replicon: chromosome
• Accession: NZ_CP076686
• Organism: Marinobacter adhaerens strain HP15-B

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A4Z1BUV4
• Locus tag: KQ249_RS04025
• Protein ID: WP_011786639.1
• Coordinates: 908512..908790 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KQ249_RS04030
• Protein ID: WP_041646003.1
• Coordinates: 908803..909117 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQ249_RS04015 (KQ249_04015)	905600..906211	-	612	WP_041646001.1	dTMP kinase	-
KQ249_RS04020 (KQ249_04020)	906255..907715	+	1461	WP_014579268.1	radical SAM protein	-
KQ249_RS04025 (KQ249_04025)	908512..908790	+	279	WP_011786639.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KQ249_RS04030 (KQ249_04030)	908803..909117	+	315	WP_041646003.1	HigA family addiction module antitoxin	Antitoxin
KQ249_RS04035 (KQ249_04035)	909279..909893	+	615	WP_014579271.1	tyrosine-type recombinase/integrase	-
KQ249_RS04040 (KQ249_04040)	910121..911290	+	1170	WP_216688054.1	MerR family transcriptional regulator	-
KQ249_RS04045 (KQ249_04045)	911452..911904	+	453	WP_041646005.1	hypothetical protein	-
KQ249_RS04050 (KQ249_04050)	912201..912686	+	486	WP_041646007.1	DUF3592 domain-containing protein	-
KQ249_RS04055 (KQ249_04055)	912870..913337	+	468	WP_041646010.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	884588..917008	32420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10765.21 Da
• Isoelectric Point: 8.5359

>T206204 WP_011786639.1 NZ_CP076686:908512-908790 [Marinobacter adhaerens]
MIKSFKCKHTEELVKTGKTRRFNAIQRTAERKLQQLLSAEALKDLKSPPGNRLEPLSGDREGQHSIRINDQWRLCFRWTD
EGAEDVEIVDYH

Nucleotide

• Length: 279 bp

>T206204 NZ_CP102192:355289-355636 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11975.75 Da
• Isoelectric Point: 8.5038

>AT206204 WP_041646003.1 NZ_CP076686:908803-909117 [Marinobacter adhaerens]
MVTVGMRPIHPGEILLEDYLEPLGMSVNALSKELYVPAQRLNEIVRERRGVSADTAMRLARYFGTTEQFWLNLQTDYDLR
RAREEKADQINKLITPRKPGHHAA

Nucleotide

• Length: 315 bp

>AT206204 NZ_CP102192:355636-355935 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Toxin

• AlphaFold DB: A0A4Z1BUV4