Detailed information of TA system TA206202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4876833..4877428
• Replicon: chromosome
• Accession: NZ_CP076679
• Organism: Rhodopseudomonas palustris strain VNW64

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B3Q7W1
• Locus tag: KQX61_RS22635
• Protein ID: WP_012497552.1
• Coordinates: 4876833..4877111 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KQX61_RS22640
• Protein ID: WP_012497553.1
• Coordinates: 4877123..4877428 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQX61_RS22615 (KQX61_22520)	4872147..4872503	-	357	WP_264053388.1	hypothetical protein	-
KQX61_RS22620 (KQX61_22525)	4872621..4875689	+	3069	WP_264053389.1	excinuclease ABC subunit UvrB	-
KQX61_RS22625 (KQX61_22530)	4875710..4876432	+	723	WP_264053390.1	hypothetical protein	-
KQX61_RS22630	4876481..4876792	-	312	Protein_4479	(2Fe-2S) ferredoxin domain-containing protein	-
KQX61_RS22635 (KQX61_22535)	4876833..4877111	+	279	WP_012497552.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KQX61_RS22640 (KQX61_22540)	4877123..4877428	+	306	WP_012497553.1	HigA family addiction module antitoxin	Antitoxin
KQX61_RS22645 (KQX61_22545)	4877471..4877641	+	171	WP_264053391.1	hypothetical protein	-
KQX61_RS22650 (KQX61_22550)	4877730..4878050	-	321	WP_264053392.1	(2Fe-2S) ferredoxin domain-containing protein	-
KQX61_RS22655 (KQX61_22555)	4878445..4879698	-	1254	WP_264053393.1	acetyl-CoA C-acetyltransferase	-
KQX61_RS22660 (KQX61_22560)	4879912..4880367	+	456	WP_264053394.1	hypothetical protein	-
KQX61_RS22665 (KQX61_22565)	4880851..4881282	+	432	WP_107356889.1	nuclear transport factor 2 family protein	-
KQX61_RS22670 (KQX61_22570)	4881490..4881930	+	441	WP_107345926.1	hypothetical protein	-
KQX61_RS22675 (KQX61_22575)	4882073..4882219	-	147	WP_199851768.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10913.43 Da
• Isoelectric Point: 9.0805

>T206202 WP_012497552.1 NZ_CP076679:4876833-4877111 [Rhodopseudomonas palustris]
MIRSFRNPATARLFADEDVPRFRAIERQARRKLLLLDGAAKLDDLRQPPGNRLEALKGDRRGQYSIRINDQWRICFEWRE
DGAEMVEIVDYH

Nucleotide

• Length: 279 bp

>T206202 NZ_CP102191:4680-4946 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11312.14 Da
• Isoelectric Point: 10.2943

>AT206202 WP_012497553.1 NZ_CP076679:4877123-4877428 [Rhodopseudomonas palustris]
MAKPKQLPPIPPGEILIEEFMRPNGISQNRLARDIDINPARVNDIVHGRSAITAAVALRLAKYFGTTPELWMNLQASYDL
RRARAADWPAIEPRVRVLAAE

Nucleotide

• Length: 306 bp

>AT206202 NZ_CP102191:4472-4696 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A2R4GZL3