Detailed information of TA system TA206197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4736729..4737324
• Replicon: chromosome
• Accession: NZ_CP076677
• Organism: Rhodopseudomonas palustris strain PP803

Toxin (Protein)

• Gene name: graT
• Uniprot ID: B3Q7W1
• Locus tag: KQX63_RS21825
• Protein ID: WP_012497552.1
• Coordinates: 4736729..4737007 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KQX63_RS21830
• Protein ID: WP_012497553.1
• Coordinates: 4737019..4737324 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KQX63_RS21815 (KQX63_21725)	4733131..4733487	-	357	WP_263989639.1	hypothetical protein	-
KQX63_RS21820 (KQX63_21730)	4733605..4736670	+	3066	WP_263989640.1	excinuclease ABC subunit UvrB	-
KQX63_RS21825 (KQX63_21735)	4736729..4737007	+	279	WP_012497552.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KQX63_RS21830 (KQX63_21740)	4737019..4737324	+	306	WP_012497553.1	HigA family addiction module antitoxin	Antitoxin
KQX63_RS21835 (KQX63_21745)	4737368..4737613	+	246	WP_263989641.1	transcriptional regulator	-
KQX63_RS21840 (KQX63_21750)	4737628..4737948	-	321	WP_263989642.1	(2Fe-2S) ferredoxin domain-containing protein	-
KQX63_RS21845 (KQX63_21755)	4738360..4739613	-	1254	WP_263989643.1	acetyl-CoA C-acetyltransferase	-
KQX63_RS21850 (KQX63_21760)	4739827..4740264	+	438	WP_263989644.1	hypothetical protein	-
KQX63_RS21855 (KQX63_21765)	4740749..4741180	+	432	WP_107345925.1	nuclear transport factor 2 family protein	-
KQX63_RS21860 (KQX63_21770)	4741388..4741828	+	441	WP_107345926.1	hypothetical protein	-
KQX63_RS21865 (KQX63_21775)	4741970..4742116	-	147	WP_199851768.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-70)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10913.43 Da
• Isoelectric Point: 9.0805

>T206197 WP_012497552.1 NZ_CP076677:4736729-4737007 [Rhodopseudomonas palustris]
MIRSFRNPATARLFADEDVPRFRAIERQARRKLLLLDGAAKLDDLRQPPGNRLEALKGDRRGQYSIRINDQWRICFEWRE
DGAEMVEIVDYH

Nucleotide

• Length: 279 bp

>T206197 NZ_CP102188:c119839-119534 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11312.14 Da
• Isoelectric Point: 10.2943

>AT206197 WP_012497553.1 NZ_CP076677:4737019-4737324 [Rhodopseudomonas palustris]
MAKPKQLPPIPPGEILIEEFMRPNGISQNRLARDIDINPARVNDIVHGRSAITAAVALRLAKYFGTTPELWMNLQASYDL
RRARAADWPAIEPRVRVLAAE

Nucleotide

• Length: 306 bp

>AT206197 NZ_CP102188:c120059-119841 [Klebsiella pneumoniae]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACCACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2R4GZL3