Detailed information of TA system TA206145

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1894784..1895286
• Replicon: chromosome
• Accession: NZ_CP076654
• Organism: Escherichia coli strain NC19

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: KPE96_RS09135
• Protein ID: WP_000767829.1
• Coordinates: 1895032..1895286 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: KPE96_RS09130
• Protein ID: WP_001259255.1
• Coordinates: 1894784..1895035 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPE96_RS09105 (1889961)	1889961..1891028	-	1068	WP_000080076.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
KPE96_RS09110 (1891028)	1891028..1892098	-	1071	WP_000108941.1	histidinol-phosphate transaminase	-
KPE96_RS09115 (1892095)	1892095..1893399	-	1305	WP_001317939.1	histidinol dehydrogenase	-
KPE96_RS09120 (1893405)	1893405..1894304	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
KPE96_RS09125 (1894450)	1894450..1894500	-	51	WP_001364200.1	his operon leader peptide	-
KPE96_RS09130 (1894784)	1894784..1895035	+	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
KPE96_RS09135 (1895032)	1895032..1895286	+	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
KPE96_RS09140 (1895369)	1895369..1896193	+	825	WP_000754758.1	SDR family oxidoreductase	-
KPE96_RS09145 (1896239)	1896239..1897168	+	930	WP_000803351.1	LysR substrate-binding domain-containing protein	-
KPE96_RS09150 (1897383)	1897383..1897445	+	63	WP_010723108.1	membrane protein YoeI	-
KPE96_RS09155 (1897435)	1897435..1898793	+	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
KPE96_RS09160 (1898972)	1898972..1900030	+	1059	WP_000492346.1	thiosulfate utilization transporter TsuA/YeeE	-
KPE96_RS09165 (1900044)	1900044..1900271	+	228	WP_000985273.1	thiosulfate utilization sulfurtransferase TsuB/YeeD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T206145 WP_000767829.1 NZ_CP076654:1895032-1895286 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T206145 NZ_CP102154:597312-597581 [Streptococcus suis]
ATGGCTTATAAGCTTGTTCTCAGCGATGATGCTTTGAAGCAGTTGAAAAAAATGGACAGGCATGTGGGGATGATGCTTGC
TAAGGATTTGAAAAAACGTTTGGACGGTTTGGAGAATCCAAGACAATTTGGGAAGGCCTTGGTAGGAGACTATAAGGGGC
TGTGGCGGTATAGAGTGGGGAATTACCGTGTAATTTGTGATATTATTGATAACAAAATGGTCATCCTGGCCCTTGAAATC
GGGCACCGGAAAGAAATCTATAAAAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT206145 WP_001259255.1 NZ_CP076654:1894784-1895035 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT206145 NZ_CP102154:597095-597322 [Streptococcus suis]
ATGGGAATTGTATCCTTGCGATTAAACGATGCAGAAGAAGAAATTTTCAGAAGCTATGCTATTCATACAGGAAAAAGCCT
GTCTGAGCTATTCAAAACTGCACTTGCTGAACAGATAGAAGACCAGTTGGACTATGAAATTGGTATCAAGGCTTTGAAGA
AATTCCAAGAAAATCCTGTTACACACTCGATTGATGATGTTCTTGCGGAGTTAGAAGATGGCTTATAA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0