Detailed information of TA system TA206143

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1162024..1162751
• Replicon: chromosome
• Accession: NZ_CP076654
• Organism: Escherichia coli strain NC19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: KPE96_RS05720
• Protein ID: WP_000547564.1
• Coordinates: 1162024..1162335 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KPE96_RS05725
• Protein ID: WP_000126294.1
• Coordinates: 1162332..1162751 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPE96_RS05690 (1157166)	1157166..1158875	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
KPE96_RS05695 (1158885)	1158885..1159427	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
KPE96_RS05700 (1159427)	1159427..1160194	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
KPE96_RS05705 (1160191)	1160191..1160601	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
KPE96_RS05710 (1160594)	1160594..1161064	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
KPE96_RS05715 (1161089)	1161089..1161850	+	762	WP_001026446.1	hypothetical protein	-
KPE96_RS05720 (1162024)	1162024..1162335	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
KPE96_RS05725 (1162332)	1162332..1162751	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
KPE96_RS05730 (1162865)	1162865..1164289	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
KPE96_RS05735 (1164298)	1164298..1165755	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
KPE96_RS05740 (1166015)	1166015..1167025	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
KPE96_RS05745 (1167174)	1167174..1167701	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T206143 WP_000547564.1 NZ_CP076654:1162024-1162335 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T206143 NZ_CP102152:c1966547-1966290 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCCAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT206143 WP_000126294.1 NZ_CP076654:1162332-1162751 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT206143 NZ_CP102152:c1966809-1966549 [Streptococcus suis]
ATGGAAGCTATTGTATATTCCCATTTTCGAAATCACTTAAAGGACTATATGAAAAAGGTCAATGACGAGTTTGAACCTTT
GGTCGTGGTTAATAAAAATCCAGAGGAAGATATTGTTGTACTCTCAAAGAGTGAGTGGGATAGTTTACAAGAAACCCTCG
CTGTTGCTCGGAACACTTATCTGTCTCAAAAAGTCCTACGTGGTATGGCTAAAGTCAAAACGGGACAAACCCAAGAACGA
AACCTGATAGAGGCGGACTAA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5