Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 599535..600334 Replicon chromosome
Accession NZ_CP076654
Organism Escherichia coli strain NC19

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag KPE96_RS02925 Protein ID WP_000347251.1
Coordinates 599535..599999 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag KPE96_RS02930 Protein ID WP_001307405.1
Coordinates 599999..600334 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KPE96_RS02895 (594536) 594536..594970 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
KPE96_RS02900 (594988) 594988..595866 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
KPE96_RS02905 (595856) 595856..596635 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
KPE96_RS02910 (596646) 596646..597119 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
KPE96_RS02915 (597142) 597142..598422 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
KPE96_RS02920 (598671) 598671..599480 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
KPE96_RS02925 (599535) 599535..599999 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
KPE96_RS02930 (599999) 599999..600334 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
KPE96_RS02935 (600483) 600483..602054 - 1572 WP_048943282.1 galactarate dehydratase -
KPE96_RS02940 (602429) 602429..603763 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
KPE96_RS02945 (603779) 603779..604549 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T206138 WP_000347251.1 NZ_CP076654:c599999-599535 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T206138 NZ_CP102149:2241301-2241564 [Streptococcus suis]
ATGAGCTATAAGGTACGCTTGACCCCAAAGGCAAATAAGCAACTCAGAAAATTAGATGCCTATACTCGTAATACCATTTT
GACCTGGATTAAGAAAAATCTATACCAGACCGACAACCCACGTCTGCATGGCAAGGGATTGACAGCTAATCGATCAGGAG
CATGGCGTTATCGTGTAGGAAATTATCGGATTTTGGCAAATATTTACGATGATGAAATTGTCATTGAAGTGTTCGCCATC
GGTCACCGCAGAGATAGTTATTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT206138 WP_001307405.1 NZ_CP076654:c600334-599999 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT206138 NZ_CP102149:2241089-2241304 [Streptococcus suis]
ATGACTACTATGACGTTACGGATTAACGATGAGGATTCTAAACTGATTAAGAACTATATCCAGTTGCATGGGCTGACGAT
TTCAGAGTTTGCGCGGCAGGCTATGTTGGAACGTATCGAGGATGAATATGATTTGGCTGTTCTTCGTCAGGCTATGGCAG
AAGACGATGGGGTGCGTATTCCTATCGAAGATCTATTCGAGGAGTTTGGTATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References