Detailed information of TA system TA206108

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96865..97129
• Replicon: plasmid pNDM33-2
• Accession: NZ_CP076647
• Organism: Escherichia coli strain GD33

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: KP013_RS23200
• Protein ID: WP_001303307.1
• Coordinates: 96977..97129 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	96865..96927 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KP013_RS23185 (92967)	92967..94037	-	1071	WP_181725925.1	IncI1-type conjugal transfer protein TrbB	-
KP013_RS23190 (94056)	94056..95264	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
- (95444)	95444..95504	-	61	NuclAT_1	-	-
- (95444)	95444..95504	-	61	NuclAT_1	-	-
- (95444)	95444..95504	-	61	NuclAT_1	-	-
- (95444)	95444..95504	-	61	NuclAT_1	-	-
KP013_RS23195 (95571)	95571..96656	-	1086	WP_000080543.1	protein finQ	-
- (96865)	96865..96927	-	63	NuclAT_0	-	Antitoxin
- (96865)	96865..96927	-	63	NuclAT_0	-	Antitoxin
- (96865)	96865..96927	-	63	NuclAT_0	-	Antitoxin
- (96865)	96865..96927	-	63	NuclAT_0	-	Antitoxin
KP013_RS23200 (96977)	96977..97129	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
KP013_RS23205 (97201)	97201..97452	-	252	WP_001291968.1	hypothetical protein	-
- (97839)	97839..97890	-	52	NuclAT_2	-	-
- (97839)	97839..97890	-	52	NuclAT_2	-	-
- (97839)	97839..97890	-	52	NuclAT_2	-	-
- (97839)	97839..97890	-	52	NuclAT_2	-	-
KP013_RS23210 (98376)	98376..98552	-	177	WP_001054900.1	hypothetical protein	-
KP013_RS23215 (98761)	98761..98970	-	210	WP_001140545.1	hemolysin expression modulator Hha	-
KP013_RS23220 (99068)	99068..99682	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
KP013_RS23225 (99758)	99758..101926	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	ant(3'')-Ia / floR / mph(A) / erm(B)	-	1..113068	113068

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T206108 WP_001303307.1 NZ_CP076647:96977-97129 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T206108 NZ_CP102140:c1966198-1965941 [Streptococcus suis]
ATGGGAATCCATTTTACAGACGACGCCTGGGAAGACTATCTTTATTGGCAGGGTCAGGATAAGAAAACACTGAAGAGAAT
CAATAAGATTATCAAGGATATGCAGCGACATCCTTTTGAAGGCATTGCCAAACCAGAACCTTTGAAGTATGATTATCAAG
GTGCCTGGTCTCGGAGAATTGATGCGGAGAATCGGTTGATTTATATGGTGGAAGCTGATCGGTTGTGCATCCTGTCGCTA
AAGGATCATTATAGGTGA

Antitoxin

Nucleotide

• Length: 63 bp

>AT206108 NZ_CP076647:c96927-96865 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin