Detailed information of TA system TA206105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3741015..3741709
• Replicon: chromosome
• Accession: NZ_CP076646
• Organism: Escherichia coli strain GD33

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: KP013_RS18165
• Protein ID: WP_001263493.1
• Coordinates: 3741015..3741413 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: KP013_RS18170
• Protein ID: WP_000554757.1
• Coordinates: 3741416..3741709 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3736675)	3736675..3736755	-	81	NuclAT_11	-	-
- (3736675)	3736675..3736755	-	81	NuclAT_11	-	-
- (3736675)	3736675..3736755	-	81	NuclAT_11	-	-
- (3736675)	3736675..3736755	-	81	NuclAT_11	-	-
KP013_RS18135 (3736015)	3736015..3737259	-	1245	WP_000189541.1	esterase FrsA	-
KP013_RS18140 (3737351)	3737351..3737809	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
KP013_RS18145 (3738070)	3738070..3739527	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
KP013_RS18150 (3739584)	3739584..3740105	-	522	Protein_3561	peptide chain release factor H	-
KP013_RS18155 (3740104)	3740104..3740307	-	204	Protein_3562	RNA ligase RtcB family protein	-
KP013_RS18160 (3740553)	3740553..3741005	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
KP013_RS18165 (3741015)	3741015..3741413	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
KP013_RS18170 (3741416)	3741416..3741709	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
KP013_RS18175 (3741761)	3741761..3742816	-	1056	WP_063501914.1	DNA polymerase IV	-
KP013_RS18180 (3742887)	3742887..3743672	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
KP013_RS18185 (3743644)	3743644..3745356	+	1713	Protein_3568	flagellar biosynthesis protein FlhA	-
KP013_RS18190 (3745580)	3745580..3746077	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T206105 WP_001263493.1 NZ_CP076646:c3741413-3741015 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T206105 NZ_CP102140:c1041726-1040932 [Streptococcus suis]
ATGCGGTTAGAAGAATTTAGCAAAGATGACTTTGACTTAGCCTTAAAACGAACTATTCGTTCCTTAACTCGTGGTAAAAC
GACATCAGTTACCCCAAAAGCCATCTTACTTGGTGGGCAAAGTGGAGCAGGAAAAACAACGATACACCGTATCAAGCAGA
AAGAGTTCCAAGGTAATATTATCATTATTGACGGTGATAGCTACCGCTCTTTACACCCGAATTATCTAACTTTACAAGAA
GAGTACGGGAAGGATAGCGTGGATTATACCAAAGGTTTTGCAGGAAAAATGGTGGAACAGCTTGTGGATGAACTCAGTAA
ACAAGGTTATCATTTGTTAATCGAAGGAACTTTGAGAACGACAGAAGTCCCACGAAAAACAGCACGGCTATTGAAAGCGA
GAGGTTATCAAGTCTCATTAGCTTTGATAGCAACCAAGCCTGAATTGTCTTATCTCAGCACCTTAATCCGCTACGAAGAA
GTCTATGTGGTTAATCCAAACCAAGCTAGAGCAACTCCTAAAGAACATCATGATGGAATTGTCAATCACTTGGTTGATAA
CTTACGAGAGCTAGAAAACGACCATCTCTTTGATTATATTCAAATTTATCAAAGAGATAGAAGTTGTGTTTATGATTCGG
AAACTGATGACGGTTCAGCAGCAGAAGTGCTACAAGAATGCCTCTTCGGAAAATGGAGCAAGGTGGAGGAAGAGATGATG
AAGATGGGACAGGAATTGTTGATGGAGTTTTTGGAAATGAAAAAAGGTAGAATTTATGAAAATAAAAAAAATTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT206105 WP_000554757.1 NZ_CP076646:c3741709-3741416 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT206105 NZ_CP102140:c1042202-1041726 [Streptococcus suis]
ATGATTGGAGATAATATTAAATCGTTACGTAAAACCCATGATTTAACACAACCAGAATTCGCAAAGATTGTTGGAATTTC
TCGAAATAGTTTGAGCCGTTATGAAAATGGGACAAGCTCGTTATCAACAGAGCTCATAGACCGTATCTGTCAGAAATTTA
ATGTATCATATGTTGATATTGTAGGAGAAGAAAAAATGTTAACACCAGTGGAAGATTATCAATTAACCTTAAAAATTGAA
GTTATCAAAGAGCGTGGCGCAGCGATTTTGTCCGAACTATATCGCTATCAAGATAGTCAAGGAATTACTTTTGATGATGA
GACTAGCCCTTGGATTTTGATGAGTGATGATTTGTCTGATCTTATCAATACCAAGATTTACCTTGTCAATACTTTTGATG
AAGTGGAGCGTTACAATGGTTATTTAGATGGTATTGAGCGAATGTTGGAGCTTGCTAATCATCAGGTGGTGGCTTAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6