Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3956173..3956867 Replicon chromosome
Accession NZ_CP076645
Organism Escherichia coli strain MSI001

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag KP725_RS19405 Protein ID WP_001263491.1
Coordinates 3956173..3956571 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag KP725_RS19410 Protein ID WP_000554755.1
Coordinates 3956574..3956867 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP725_RS19380 (3951537) 3951537..3951995 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
KP725_RS19385 (3952256) 3952256..3953713 + 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
KP725_RS19390 (3953770) 3953770..3954384 - 615 WP_000602125.1 peptide chain release factor H -
KP725_RS19395 (3954381) 3954381..3955520 - 1140 WP_000521583.1 RNA ligase RtcB family protein -
KP725_RS19400 (3955711) 3955711..3956163 - 453 WP_001059882.1 GNAT family N-acetyltransferase -
KP725_RS19405 (3956173) 3956173..3956571 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
KP725_RS19410 (3956574) 3956574..3956867 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
KP725_RS19415 (3956919) 3956919..3957974 - 1056 WP_001226154.1 DNA polymerase IV -
KP725_RS19420 (3958045) 3958045..3958830 - 786 WP_000207556.1 putative lateral flagellar export/assembly protein LafU -
KP725_RS19425 (3958802) 3958802..3960514 + 1713 Protein_3829 flagellar biosynthesis protein FlhA -
KP725_RS19430 (3960630) 3960630..3961127 - 498 WP_001570960.1 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T206083 WP_001263491.1 NZ_CP076645:c3956571-3956173 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T206083 NZ_CP102135:c985961-985695 [Streptococcus suis]
ATGAAGACCTGTTATAAACTCGTACCAACCAGTCGTTTTATCAAACAGTTGAAAAAGTTGGACAAATTTACCCAAAAACA
GATTACCAACTACCTGTCCAGTCATGTGACCGACAACCCCAGACAATACGGAAAAGCCTTGACTGCCAATCGTTCTGGCC
AATGGCGATACCGTATCGGCAATTACAGGGTCATCGTCAGCATTGAGGATGATAAATTGATCGTAACAGCCCTTGAAGTC
GGGCATCGGAAAGAAATATATAAATAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT206083 WP_000554755.1 NZ_CP076645:c3956867-3956574 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT206083 NZ_CP102135:c986196-985945 [Streptococcus suis]
ATGGCAGCTATCACATTGAAAGTTTCTGAGCAGGACAAACTCTTTATGCAGGCTATGGCAAAGTTTGAAGGTGTGAGTTT
GTCAGAACTCATTCGGACCAAAACACTTGAAGCACTTGAAGATGAATACGACGCCCGTGTGGCAGATATCGCTCTAGCTG
AATACGAGGCAGACTTGGCAAATGGAATTGAGCCGATTAGCTGGGAGGAAATGTTGGAAGAGTTGGGGTTGACGGATGAA
GACCTGTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References