Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1993495..1993997 Replicon chromosome
Accession NZ_CP076645
Organism Escherichia coli strain MSI001

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag KP725_RS09615 Protein ID WP_000767829.1
Coordinates 1993743..1993997 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag KP725_RS09610 Protein ID WP_001259255.1
Coordinates 1993495..1993746 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP725_RS09585 (1988673) 1988673..1989740 - 1068 WP_021526184.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
KP725_RS09590 (1989740) 1989740..1990810 - 1071 WP_021526185.1 histidinol-phosphate transaminase -
KP725_RS09595 (1990807) 1990807..1992111 - 1305 WP_021526186.1 histidinol dehydrogenase -
KP725_RS09600 (1992117) 1992117..1993016 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
KP725_RS09605 (1993162) 1993162..1993212 - 51 WP_001364200.1 his operon leader peptide -
KP725_RS09610 (1993495) 1993495..1993746 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
KP725_RS09615 (1993743) 1993743..1993997 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
KP725_RS09620 (1994080) 1994080..1994904 + 825 WP_000754737.1 SDR family oxidoreductase -
KP725_RS09625 (1994950) 1994950..1995123 + 174 Protein_1891 LysR family transcriptional regulator -
KP725_RS09630 (1995210) 1995210..1995860 + 651 WP_000993914.1 IS66-like element accessory protein TnpA -
KP725_RS09635 (1995860) 1995860..1996207 + 348 WP_000624622.1 IS66 family insertion sequence element accessory protein TnpB -
KP725_RS09640 (1996227) 1996227..1997798 + 1572 WP_124038510.1 IS66 family transposase -
KP725_RS09645 (1997830) 1997830..1998591 + 762 Protein_1895 LysR substrate-binding domain-containing protein -
KP725_RS09650 (1998806) 1998806..1998868 + 63 WP_010723108.1 membrane protein YoeI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T206070 WP_000767829.1 NZ_CP076645:1993743-1993997 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T206070 NZ_CP102095:856823-857659 [Mycoplasmopsis agalactiae]
ATGAATAAGAATAAATTAATAAGTCTGTGTCATAAAATTGCAGATGAAAATAGTGCAAATTTCAATGTGGTTTTAATTCA
ATATTTTATGGAAGGTGTGTTAAGAAGAATATCTAAAAGCAATGAACGAGATAATTTTGTATTTAAGGGCGGTTTTCTTT
TGTCTAACATTATGGGCTTAGATAAAAGATCCACAATGGATATTGATTTAGAGATGATAAAAGTTCAAAAAATAAGCACT
GCTAAAATAATAGAAAAATTTGACAACATATTAAAAGTTGATGAAGAGGATGGAATTAAATATCAAATCCTAAAGTATAC
CGATATTAGAAAAGAGCATAAATATAGTGGTATTAGCATATCACTGCTATGCCAAATAGAAAATATAAGACAGATTATTT
CTATAGACATTGCCTCAGGAGATGTTATTACACCCAAAGAAATTGTTTATGAATACAAAAGCATGTTTTCAAATCAGAAT
TTTTCTATTTTAGCTTATAACATTGAAACTATGCTGGCTGAAAAACTTGAAACAATATTTTCTTTTGGCTATTTTAATAC
AAGGTTTAAAGATTTTTATGATGTTTATGTTATCTATACTTTTAAAAGCAAAAATATTGATATTGATAGACTTGAAAATG
CTTGTTACAACACATTTAAAAATAGAAATAGTGAATTTAACAAACAGCAATTAATAGAGTTAATTAACAAGATTTCTTTA
AATACGTTGATGAATGAAAGATGAAATAAGTACATAAGTCAGCTAAATAACCAACAGGAAATAGACTTTAAAACTGTAAC
TGATTCAATAATGAAACTACTTGAACTAATAGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT206070 WP_001259255.1 NZ_CP076645:1993495-1993746 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT206070 NZ_CP102095:856216-856830 [Mycoplasmopsis agalactiae]
ATGGAACAAAAGCAATCGAATTCTAAATATTACAAGATAATTTATCAACTATTATATGTGAATGATGGTGTTATAACATA
TAAACAACTAAAGGAACATAATATTCCTAGTATATATTTAACAAGAATGATAAGAAAAGGCACTCTTATTAGGAAAACCA
AAGGCATTTATATTATTAATAATGGGCATTATGATGAATATTATTTCCTAAGCAAAAACTATAATTCATTGATTTTTTCT
CATTATAGTGCACTATATCTGAACAATATTATTGACATTTTTCCACACCAAATTGATGTTACGGTTTACAGTGGCTATAA
TGCAAAAGGCATTAAAAAGTATGCAAATGTTTACTACTGTGATAGAGACATACACTCAATTGGACTATCAGAAGCAAAAA
CAATATTTGGTAACAAAGTAAAGGTATATGACTTTGAGAGAACAATCTGTGATTTTGTAAAACTACGCAAAAAAACAGAT
AGTGAAATTTTTTCTAAGCTTATTGGTGCATATATTGAATATGAGAAGAAAGATCTAAAAAAATTATATGAATATGCCTA
TAAATTAGGCATACACAAAAAAATATTGGAGATATTTGAATTATTTTATGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References