Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 654626..655353 Replicon chromosome
Accession NZ_CP076645
Organism Escherichia coli strain MSI001

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag KP725_RS03200 Protein ID WP_000550189.1
Coordinates 654626..654940 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KP725_RS03205 Protein ID WP_000560266.1
Coordinates 654937..655353 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP725_RS03180 (650784) 650784..651770 - 987 WP_216684197.1 Gfo/Idh/MocA family oxidoreductase -
KP725_RS03185 (651849) 651849..652541 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
KP725_RS03190 (652618) 652618..653121 - 504 WP_001300832.1 M48 family metallopeptidase -
KP725_RS03195 (653206) 653206..654342 + 1137 WP_000018695.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
KP725_RS03200 (654626) 654626..654940 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
KP725_RS03205 (654937) 654937..655353 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
KP725_RS03210 (655398) 655398..657416 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
KP725_RS03215 (657842) 657842..660193 - 2352 WP_000695489.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T206066 WP_000550189.1 NZ_CP076645:654626-654940 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T206066 NZ_CP102094:c1403442-1403077 [Streptococcus suis]
ATGTACACTATTTATTTTTATAAGGATAAAAATGGAAGGGAGCCTGTTTTAGAGTATCTGAGGGAGCTTGCATCTAAAAA
GGGGAAAGATAGCAGGATTAAATTGAATAAGATAAATGATTATATTGAACTTTTAAGTCAACTGGGAACACAAATTGGTG
AACCATATGTGAAGCACTTGGAAGGTAATATTTGGGAATTGCGTCCCTTAAGAGATAGGATTTTATTTGTCGCATGGTAT
GAAGGGAGTTTTGTTTTACTGCATCATTTTGTGAAACGGTCACAGAAAACACCACGGAGGGAGCTTGAAAAGGCTCAACG
TGAGCTGGCAGACTTGAAAGAAAGGGGTATTAACAATGGAAAATAG

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT206066 WP_000560266.1 NZ_CP076645:654937-655353 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT206066 NZ_CP102094:c1403087-1402788 [Streptococcus suis]
ATGGAAAATAGTGCAATTGGTAGTAACTGGCAATCGGTACGGTCTGAACTCTTCAGTGAGAAGGAAATCATAGAAAGTGA
TTTGCGTGTGGCAATTATGAGCGAACTGATAAAAGCAAGGCATGAACAGGGAGTAAGTCAGAAAAGACTAGAGGAGCTAA
GCGGGGTGAGTCAACCAGTTATTGCAAGAATGGAAACAGGTAAAACTAGTCCACAATTGGACACTGTCTTGAAGGTATTG
GCTAGTCTGGGAAAAACTTTAGCAGTTGTGCCTTTGAAACAGGAACGACATTTTCAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References