Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 42113..42840 Replicon plasmid unnamed
Accession NZ_CP076606
Organism Raoultella ornithinolytica strain XF201

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LNL2
Locus tag KP007_RS25940 Protein ID WP_004118600.1
Coordinates 42529..42840 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID V0AHC4
Locus tag KP007_RS25935 Protein ID WP_000990392.1
Coordinates 42113..42532 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KP007_RS25905 (KP007_25905) 37800..38162 - 363 WP_020324593.1 arsenite efflux transporter metallochaperone ArsD -
KP007_RS25910 (KP007_25910) 38210..38563 - 354 WP_001114073.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KP007_RS26815 38856..38996 + 141 WP_004118614.1 hypothetical protein -
KP007_RS26820 39164..39472 + 309 WP_020324596.1 Ref family recombination enhancement nuclease -
KP007_RS25920 (KP007_25920) 39896..40555 + 660 WP_000078540.1 hypothetical protein -
KP007_RS25925 (KP007_25925) 40552..40881 + 330 WP_004118613.1 hypothetical protein -
KP007_RS25930 (KP007_25930) 40874..42076 + 1203 WP_003031541.1 hypothetical protein -
KP007_RS25935 (KP007_25935) 42113..42532 - 420 WP_000990392.1 helix-turn-helix domain-containing protein Antitoxin
KP007_RS25940 (KP007_25940) 42529..42840 - 312 WP_004118600.1 type II toxin-antitoxin system HigB family toxin Toxin
KP007_RS25945 (KP007_25945) 43051..43431 + 381 Protein_55 gluconate permease -
KP007_RS25950 (KP007_25950) 43481..44176 - 696 WP_004118595.1 lactate utilization protein C -
KP007_RS25955 (KP007_25955) 44169..45596 - 1428 WP_000023895.1 LutB/LldF family L-lactate oxidation iron-sulfur protein -
KP007_RS25960 (KP007_25960) 45607..46326 - 720 WP_001102107.1 (Fe-S)-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(D) / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-14 / aac(3)-IId / blaLAP-2 / qnrS1 / sul1 / qacE / dfrA1 / tet(A) - 1..185066 185066


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-136)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12386.14 Da        Isoelectric Point: 10.0935

>T206051 WP_004118600.1 NZ_CP076606:c42840-42529 [Raoultella ornithinolytica]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Download         Length: 312 bp

>T206051 NZ_CP102086:3820497-3821840 [Proteus mirabilis]
ATGAAAGCTCAAATACTATCTGTTGCTATGAATGGTGATTTGGTTGGTAAACTAACCAAAAATACTGATGGTGGGATGTC
ATTTCAATACGCAGAAGAATGGTTGGCTGAACCAGGAGCGCGGGCAATCTCATTATCACTTCCTTTGCAAAAGGGGCGCA
TAATCGGTAGCGCAGTTTACAATTTCTTCAGCAACCTTTTACCTGATTCTGAGGCAATTATTGATAGAATGCAGATTCGT
TTTAAGGTAAAAACAAACCATCCTTTTGATCTGCTAGCCGCTGTAGGTCGTGACTGTGTTGGCGCAATCCAATTATATTC
TCCTGATACTGAAATTCCATCGGTGATGGAAACCCATGCTGAACCTTTAAATGAAAATCAAATCGAAGAGCTTTTAGACG
GTTATCAAACCGCCCCCCTTGGAATGTCCGAGGATGTAGACTTTCGGATCTCCCTTGCAGGGGCTCAAGAGAAAACAGCA
TTACTTTGGTATCAAGACTGTTGGCAGCGACCACAAGGCAGTACCCCGACAAGTCATATTTTCAAATTGCCCATAGGTCT
GATTGAACACCATAATATTGATCTGCGCGAAAGCTGTGAAAACGAATGGCTATGCTTGTGTATAGCAAGAGAATTTGGAT
TTCCAGTCACCAACGCTGAATTGGCAATATTCGGTCAGAAAAAGGTACTGATCGTAGAGCGTTTTGACCGAAAATGGTCC
TCATCTGGGAACTGGCTGTTGCGCCTTCCCCAAGAGGATTGCTGTCAGGCTTTGAGCGTTGCCCCTGCATTAAAATATGA
ATCTGATGGTGGTCCAGGTATTGAATCTATCATGACGTTGCTTTTGGGCTCACAAAAAGCCATCGACGATAGAGAAATGT
TTTTTAAATCACAAATTCTGTTCTGGATGCTGGCCGCTATAGATGGGCATGGGAAAAACTTTTCCATCTTCATTGAATCT
GGAACATCTTTTCGTTTAACGCCCTTGTATGATGTTATGTCTGCTTTCCCAATTTTTGAAGCACGAGGAATTGAGATAAA
AAAGGCCAAAATGGCTATGGCATTACAAGGAAAAAACAGACAATACAATTTTTCCATGATACAACCTCGTCATTTTATCT
CTACCGCTACAAAGGTCGGGTTTCCAACAAAAACCGCAATGGAGCTAATGGTTGATATGGCCGAAAAGACTGATGCAGTT
ATAGCTACCGTATCATCTCAACTACCTGCGGACTTTCCTGAGCATATCAGTAACGCCATATTTAGTGGGTTATCAATACA
AGCCGATAAGATTAAAAGATGGTCTTCAGGAACGAGCTCACAAAATATGATGGTTTCGAATTGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15534.71 Da        Isoelectric Point: 4.4702

>AT206051 WP_000990392.1 NZ_CP076606:c42532-42113 [Raoultella ornithinolytica]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Download         Length: 420 bp

>AT206051 NZ_CP102086:3820242-3820493 [Proteus mirabilis]
ATGAAAATTACAACGCCATCAATGTTAGCCGCTGCAATACATGACTTGCGCAAACAAAGTAAATTAACCCAAGCTCAAAC
GGCTGAACAAGTCGGTCTCAGGCAAGCAACTGTCTCTGAATTTGAGAACAACCCAGAATCAACAAAACTCGAAACGCTTT
TCAAAATCCTTGCCTCTTTGGAACTGGAACTACATGTAGTGAAGCGAGGAAAAAACCTGAGTGAGGGTAAGGTTTGGAAT
AAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNL2

Antitoxin

Source ID Structure
AlphaFold DB V0AHC4

References