Detailed information of TA system TA206033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 19236..19887
• Replicon: plasmid unnamed
• Accession: NZ_CP076604
• Organism: Acinetobacter schindleri strain MCDA01

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: KMZ14_RS15290
• Protein ID: WP_004668307.1
• Coordinates: 19531..19887 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KMZ14_RS15285
• Protein ID: WP_001140620.1
• Coordinates: 19236..19538 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KMZ14_RS15265 (KMZ14_15205)	14746..15177	-	432	WP_005173624.1	hypothetical protein	-
KMZ14_RS15270 (KMZ14_15210)	15962..17827	+	1866	WP_273799128.1	copper resistance system multicopper oxidase	-
KMZ14_RS15275 (KMZ14_15215)	17814..18152	+	339	WP_004897649.1	hypothetical protein	-
KMZ14_RS15280 (KMZ14_15220)	18139..19083	+	945	WP_104510421.1	copper resistance protein B	-
KMZ14_RS15285 (KMZ14_15225)	19236..19538	-	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
KMZ14_RS15290 (KMZ14_15230)	19531..19887	-	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KMZ14_RS15295 (KMZ14_15235)	20433..20874	-	442	Protein_21	winged helix-turn-helix transcriptional regulator	-
KMZ14_RS15300 (KMZ14_15240)	21044..21739	+	696	WP_273799129.1	AzlC family ABC transporter permease	-
KMZ14_RS15305 (KMZ14_15245)	21732..22043	+	312	WP_262451135.1	AzlD family protein	-
KMZ14_RS15310 (KMZ14_15250)	22148..22393	+	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KMZ14_RS15315 (KMZ14_15255)	22383..22676	+	294	WP_004897647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KMZ14_RS15320 (KMZ14_15260)	22754..24079	-	1326	WP_273799130.1	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..166835	166835
	-	flank	IS/Tn	-	-	22754..24091	1337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T206033 WP_004668307.1 NZ_CP076604:c19887-19531 [Acinetobacter schindleri]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T206033 NZ_CP102077:1472571-1473053 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT206033 WP_001140620.1 NZ_CP076604:c19538-19236 [Acinetobacter schindleri]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT206033 NZ_CP102077:1472317-1472583 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: N9NM16