Detailed information of TA system TA205956

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 122590..122843
• Replicon: plasmid p19PDR22-KPC4
• Accession: NZ_CP076554
• Organism: Klebsiella pneumoniae strain 19PDR22

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HF574_RS30230
• Protein ID: WP_001312851.1
• Coordinates: 122694..122843 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	122590..122649 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF574_RS30190 (118250)	118250..118315	-	66	Protein_157	helix-turn-helix domain-containing protein	-
HF574_RS30195 (118368)	118368..119072	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
HF574_RS30200 (119097)	119097..119297	+	201	WP_072354025.1	hypothetical protein	-
HF574_RS30205 (119317)	119317..120063	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
HF574_RS30210 (120118)	120118..120678	+	561	WP_000139328.1	fertility inhibition protein FinO	-
HF574_RS30215 (120810)	120810..121010	+	201	WP_015059022.1	hypothetical protein	-
HF574_RS30220 (121396)	121396..121995	+	600	WP_032083981.1	PIN domain-containing protein	-
HF574_RS30225 (122057)	122057..122389	+	333	WP_152916585.1	hypothetical protein	-
- (122590)	122590..122649	-	60	NuclAT_1	-	Antitoxin
- (122590)	122590..122649	-	60	NuclAT_1	-	Antitoxin
- (122590)	122590..122649	-	60	NuclAT_1	-	Antitoxin
- (122590)	122590..122649	-	60	NuclAT_1	-	Antitoxin
HF574_RS30230 (122694)	122694..122843	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
HF574_RS30235 (123127)	123127..123375	+	249	WP_000083837.1	replication regulatory protein RepA	-
HF574_RS30555 (123490)	123490..123674	+	185	Protein_167	protein CopA/IncA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / rmtB / blaTEM-1B / blaSHV-12	-	1..123686	123686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T205956 WP_001312851.1 NZ_CP076554:122694-122843 [Klebsiella pneumoniae]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T205956 NZ_CP101940:c2334844-2334557 [Salmonella enterica subsp. enterica]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGTTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCCCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT205956 NZ_CP076554:c122649-122590 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin