Detailed information of TA system TA205952

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28618..28887
• Replicon: plasmid p19PDR22-KPC4
• Accession: NZ_CP076554
• Organism: Klebsiella pneumoniae strain 19PDR22

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HF574_RS29605
• Protein ID: WP_001372321.1
• Coordinates: 28762..28887 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	28618..28683 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF574_RS29580	24328..24855	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HF574_RS29585	24913..25146	+	234	WP_000006003.1	DUF905 family protein	-
HF574_RS29590	25207..27230	+	2024	Protein_34	ParB/RepB/Spo0J family partition protein	-
HF574_RS29595	27299..27733	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HF574_RS29600	27730..28449	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	28461..28685	+	225	NuclAT_0	-	-
-	28461..28685	+	225	NuclAT_0	-	-
-	28461..28685	+	225	NuclAT_0	-	-
-	28461..28685	+	225	NuclAT_0	-	-
-	28618..28683	-	66	-	-	Antitoxin
HF574_RS30535	28671..28820	+	150	Protein_37	plasmid maintenance protein Mok	-
HF574_RS29605	28762..28887	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HF574_RS29610	29206..29502	-	297	Protein_39	hypothetical protein	-
HF574_RS29615	29802..30098	+	297	WP_001272251.1	hypothetical protein	-
HF574_RS29620	30209..31030	+	822	WP_001234445.1	DUF932 domain-containing protein	-
HF574_RS29625	31327..31974	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
HF574_RS29630	32251..32634	+	384	WP_000124981.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HF574_RS29635	32825..33511	+	687	WP_015059009.1	PAS domain-containing protein	-
HF574_RS29640	33605..33832	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCTX-M-65 / rmtB / blaTEM-1B / blaSHV-12	-	1..123686	123686

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T205952 WP_001372321.1 NZ_CP076554:28762-28887 [Klebsiella pneumoniae]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T205952 NZ_CP101938:561702-562052 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin

Nucleotide

• Length: 66 bp

>AT205952 NZ_CP076554:c28683-28618 [Klebsiella pneumoniae]
GTGGACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCT

Structures

Antitoxin