Detailed information of TA system TA205951

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13590..14247
• Replicon: plasmid p19PDR22-KPC3
• Accession: NZ_CP076553
• Organism: Klebsiella pneumoniae strain 19PDR22

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: HF574_RS28590
• Protein ID: WP_000270043.1
• Coordinates: 13897..14247 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HF574_RS28585
• Protein ID: WP_000124640.1
• Coordinates: 13590..13892 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HF574_RS28540 (HF574_29275)	9234..9662	+	429	WP_000591074.1	hypothetical protein	-
HF574_RS28545 (HF574_29280)	9719..10078	+	360	WP_000422768.1	hypothetical protein	-
HF574_RS28550 (HF574_29285)	10078..10524	+	447	WP_000919345.1	Fe3+-siderophore ABC transporter permease	-
HF574_RS28555 (HF574_29290)	10521..11039	+	519	WP_000210756.1	nitrite reductase	-
HF574_RS28560 (HF574_29295)	11039..11269	+	231	WP_000972663.1	hypothetical protein	-
HF574_RS28565 (HF574_29300)	11256..12094	+	839	Protein_17	hypothetical protein	-
HF574_RS28570 (HF574_29305)	12325..12852	+	528	WP_001236377.1	thermonuclease family protein	-
HF574_RS28575 (HF574_29310)	12910..13182	+	273	WP_001043047.1	HU family DNA-binding protein	-
HF574_RS28580 (HF574_29315)	13270..13563	+	294	WP_001239997.1	chromosome segregation protein ParM	-
HF574_RS28585 (HF574_29320)	13590..13892	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
HF574_RS28590 (HF574_29325)	13897..14247	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HF574_RS28595 (HF574_29330)	14410..14958	+	549	WP_001061574.1	transcriptional regulator	-
HF574_RS28600 (HF574_29335)	15299..15493	+	195	WP_000343597.1	hypothetical protein	-
HF574_RS28605 (HF574_29340)	15504..15875	+	372	WP_000516916.1	hypothetical protein	-
HF574_RS28610 (HF574_29345)	15868..16338	+	471	WP_001281821.1	hypothetical protein	-
HF574_RS28615 (HF574_29350)	16353..16688	-	336	WP_000683476.1	hypothetical protein	-
HF574_RS28620 (HF574_29355)	16785..17273	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
HF574_RS28625 (HF574_29360)	17276..17773	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / armA / msr(E) / mph(E) / aph(6)-Id / aph(3'')-Ib / sul2 / blaKPC-2	-	1..162180	162180

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T205951 WP_000270043.1 NZ_CP076553:c14247-13897 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T205951 NZ_CP101937:2252693-2252809 [Bacillus subtilis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT205951 WP_000124640.1 NZ_CP076553:c13892-13590 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT205951 NZ_CP101937:c2252983-2252804 [Bacillus subtilis]
AATGATACAATTATATAATTATTTTTTGCATTTTGTTTAATTAAATGCATAAAATAAAAAGACCAGGGTGTTGGTAGCAC
CCTGGCTCTGTACAAAAGCTGCCCATCAAAGGGCTTGCTCGTTGTCAAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAA
ACTCAAGGGAAGGTCTATTT

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9