Detailed information of TA system TA205936

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 29141..29405
• Replicon: plasmid pL3452210II_4
• Accession: NZ_CP076531
• Organism: Escherichia coli strain L3452210II

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: KPE21_RS24135
• Protein ID: WP_001387489.1
• Coordinates: 29141..29293 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	29345..29405 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPE21_RS24100 (24233)	24233..24766	-	534	WP_001221666.1	lipocalin family protein	-
KPE21_RS24105 (24860)	24860..26005	-	1146	WP_039023136.1	extended-spectrum class C beta-lactamase CMY-42	-
KPE21_RS24370 (27210)	27210..27308	+	99	Protein_27	ethanolamine utilization protein EutE	-
KPE21_RS24115 (27380)	27380..27589	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
KPE21_RS24120 (27981)	27981..28157	+	177	WP_001054897.1	hypothetical protein	-
KPE21_RS24125 (28222)	28222..28518	-	297	WP_011264046.1	DinQ-like type I toxin DqlB	-
KPE21_RS24130 (28818)	28818..29069	+	252	WP_001291964.1	hypothetical protein	-
KPE21_RS24135 (29141)	29141..29293	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
- (29345)	29345..29405	+	61	NuclAT_0	-	Antitoxin
- (29345)	29345..29405	+	61	NuclAT_0	-	Antitoxin
- (29345)	29345..29405	+	61	NuclAT_0	-	Antitoxin
- (29345)	29345..29405	+	61	NuclAT_0	-	Antitoxin
KPE21_RS24140 (29614)	29614..29988	+	375	WP_223349261.1	hypothetical protein	-
KPE21_RS24145 (30141)	30141..31298	+	1158	Protein_34	IS1380-like element ISEcp1 family transposase	-
KPE21_RS24150 (31354)	31354..32051	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
KPE21_RS24155 (32062)	32062..32370	-	309	WP_039023235.1	transcription termination/antitermination NusG family protein	-
KPE21_RS24160 (32812)	32812..33099	-	288	WP_000074855.1	conjugal transfer protein TraA	-
KPE21_RS24375 (33137)	33137..33295	-	159	Protein_38	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..34206	34206
	-	inside	IScluster/Tn	blaCMY-42	-	24860..31629	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T205936 WP_001387489.1 NZ_CP076531:c29293-29141 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T205936 NZ_CP101930:c1321670-1320924 [Bacillus subtilis]
ATGGTTTTATTCTTTCAGATCATGGTCTGGTGCATCGTGGCCGGACTGGGGTTATACGTGTATGCCACGTGGCGTTTCGA
AGCGAAGGTCAAAGAAAAAATGTTCGCCATCCGGAAAACTTGGTATTTGCTGTTTGTTCTGGGCGCTATGGTATACTGGA
CATATGAGCCCACTTCCCTATTTACCCACTGGGAACGGTATCTCATTGTCGCAGTCAGTTTTGCTTTGATTGATGCTTTT
ATCTTCTTAAGTGCATACGTCAAAAAACTGGCCGGCAGCGAGCTTGAAACAGACACAAGAGAAATTCTTGAAGAAAACAA
CGAAATGCTCCACATGTACCTCAATCGGCTGAAAACATACCAATACCTATTGAAAAACGAACCGATCCATGTTTATTATG
GAAGTATAGATGCTTATGCTGAAGGTATTGATAAGCTGCTGAAAACCTATGCTGATAAAATGAACTTAACGGCTTCTCTT
TGCCACTATTCGACACAGGCTGATAAAGACCGGTTAACCGAGCATATGGATGATCCGGCAGATGTACAAACACGGCTCGA
TCGAAAGGATGTTTATTACGACCAATACGGAAAAGTGGTTCTCATCCCTTTTACCATCGAGACACAGAACTATGTCATCA
AGCTGACGTCTGACAGCATTGTCACGGAATTTGATTATTTGCTATTTACGTCATTAACGAGCATATATGATTTGGTGCTG
CCAATTGAGGAGGAAGGTGAAGGATAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT205936 NZ_CP076531:29345-29405 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin