Detailed information of TA system TA205931

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4089533..4090260
• Replicon: chromosome
• Accession: NZ_CP076527
• Organism: Escherichia coli strain L3452210II

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: KPE21_RS19830
• Protein ID: WP_000547564.1
• Coordinates: 4089533..4089844 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KPE21_RS19835
• Protein ID: WP_000126294.1
• Coordinates: 4089841..4090260 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPE21_RS19800 (4084675)	4084675..4086384	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
KPE21_RS19805 (4086394)	4086394..4086936	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
KPE21_RS19810 (4086936)	4086936..4087703	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
KPE21_RS19815 (4087700)	4087700..4088110	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
KPE21_RS19820 (4088103)	4088103..4088573	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
KPE21_RS19825 (4088598)	4088598..4089359	+	762	WP_001026446.1	hypothetical protein	-
KPE21_RS19830 (4089533)	4089533..4089844	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
KPE21_RS19835 (4089841)	4089841..4090260	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
KPE21_RS19840 (4090374)	4090374..4091798	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
KPE21_RS19845 (4091807)	4091807..4093264	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
KPE21_RS19850 (4093524)	4093524..4094534	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
KPE21_RS19855 (4094683)	4094683..4095210	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T205931 WP_000547564.1 NZ_CP076527:4089533-4089844 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T205931 NZ_CP101925:c4608216-4608043 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT205931 WP_000126294.1 NZ_CP076527:4089841-4090260 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT205931 NZ_CP101925:c4608540-4608244 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5